WT-1、P53、MMP-9和TIMP-2在卵巢浆液性乳头状癌中的表达及意义  

Expression and significance of WT-1,P53,MMP-9 and TIMP-2 in ovarian papillary serous carcinoma

作　　者：杨杰斌[1] 彭真年[1] 吴伟强[1] 

机构地区：[1]重庆医科大学基础医学院病理学教研室,重庆400016

出　　处：《重庆医科大学学报》2008年第12期1495-1499,共5页Journal of Chongqing Medical University

摘　　要：目的:探讨WT-1、P53、基质金属蛋白酶-9(MMP-9)和基质金属蛋白酶组织抑制剂-2(TIMP-2)在卵巢浆液性乳头状癌中的表达及其意义。方法:采用免疫组化SP法检测WT-1、P53、MMP-9和TIMP-2在52例卵巢浆液性乳头状癌中的表达,并与15例腹膜浆液性乳头状癌和11例子宫内膜浆液性乳头状癌进行比较。结果:WT-1蛋白在卵巢浆液性乳头状癌、腹膜浆液性乳头状癌和子宫内膜浆液性乳头状癌中的阳性表达率分别为96.2%、93.3%和36.4%;P53蛋白分别为78.8%、80%和72.7%;MMP-9和TIMP-2分别为90.4%、93.3%、100%和92.3%、93.3%、100%。图像分析卵巢浆液性乳头状癌低分化组表达WT-1、P53、MMP-9和TIMP-2的面积积分光密度值(AIOD/μm2)均明显高于高分化组(P<0.01)。卵巢浆液性乳头状癌和腹膜浆液性乳头状癌表达WT-1的AIOD/μm2明显高于子宫内膜浆液性乳头状癌(P<0.01)。结论:WT-1、P53、MMP-9和TIMP-2在低分化卵巢浆液性乳头状癌中的表达高于高分化组。WT-1、P53、MMP-9和TIMP-2的过表达与卵巢浆液性乳头状癌的分化程度有关。WT-1蛋白检测可作为鉴别卵巢浆液性乳头状癌与子宫内膜浆液性乳头状癌的标记物。Objective： To investigate the expression and significance of WT-1,P53,matrix metalloproteinase-9（MMP-9） and tissue inhibitor of metalloproteinase-2（TIMP-2） in ovarian papillary serous carcinoma. Methods： WT-1,P53,MMP-9 and TIMP-2 were detected by S-P immunohistochemitry in 52 cases of ovarian papillary serous carcinoma,and compared with 15 cases of primary peritoneal papillary serous carcinoma and 11 cases of uterine papillary serous carcinoma. Results： The positive expression rates of WT-1 in ovarian papillary serous carcinoma,peritoneal papillary serous carcinoma and uterine papillary serous carcinoma were 96.2%,93.3%,and 36.4%,respectively.The positive expression rates of P53 were 78.$%,80%,and 72.7%,respectively.The positive expression rates of MMP-9 and TIMP-2 were 90.4%,93.3%,100%,and 92.3%,93.3%,100%,respectively.The area integrated optical density （AIOD/μ m2） of WT-1 ,P53,MMP-9 and TIMP-2 analyzed by image analyzer in low differentiation group of ovarian serous carcinoma were high than those in high differentiation group（P〈0.01）.The AIOD/μm2 of WT-1 in ovarian papillary serous carcinoma and primary peritoneal papillary serous carcinoma were high than that in uterine papillary serous carcinoma （P〈0.01）. Conclusion： The expression of WT-1 ,P53, MMP-9 and TIMP-2 in low differentiation group of ovarian papillary serous carcinoma are higher than those in high differentiation granp.The overexpression of WT-1 ,P53,MMP-9 and TIMP-2 are related to the degree of differentiation of the ovarian papillary serous carcinoma.The detection of WT-1 may be a useful marker for distinguishing ovarian papillary serous carcinoma from uterine papillary serous carcinoma.

关 键 词：卵巢肿瘤 浆液性乳头状癌 免疫组化 图像分析 

分 类 号：R737.31[医药卫生—肿瘤]