坛紫菜雌性叶状体的抗生素敏感性测定  

The determination of sensitivities of Porphyra haitanensis female thalloid to antibiotics

作　　者：李福后[1] 王伟霞[1] 王萍[1] 高飞[1] 

机构地区：[1]江苏省海洋生物技术重点建设实验室,淮海工学院海洋学院,江苏连云港222005

出　　处：《海洋学研究》2008年第4期111-115,共5页Journal of Marine Sciences

基　　金：江苏省海洋生物技术重点建设实验室资助项目(2005 HS011)

摘　　要：考察了坛紫菜雌性叶状体对氯霉素、卡那霉素、四环素盐酸盐和G-418这4种抗生素的敏感性。结果表明,坛紫菜雌性叶状体对各种抗生素的敏感性不同:对氯霉素敏感;对卡那霉素不敏感,有很强的耐受性;对四环素盐酸盐、G-418有一定的耐受性,但四环素盐酸盐、G-418对体细胞分裂、分化具有明显的抑制作用。研究表明氯霉素可作为坛紫菜基因工程的有效选择压力。氯霉素乙酰转移酶(CAT)基因有可能成为坛紫菜基因工程理想的选择标记基因。Sensitivities of Porphyra haitanensis female thalloid to four kinds of antibiotics were studied respectively. The thalloid was soaked in sterilized seawater for anabiosis at 20 ℃ for 1-2 d first. Then, the anabatie thalloid was spread on a piece of filter paper and the tissues differentiated by the sexual cells on the margin were removed. The thalloid was cut into 2 mm X 2 mm lumps, which were cultured in 25 mL PES medium. Different antibiotics of different mass concentrations were added into the culture medium. The cell development was observed with microscope for 18 d, and the dead tissues were recorded and analyzed. The experiment with the same concentration was done for three times, and at the same time, the experiments without adding antibiotics was done as the contrast. The results show that Porphyra haitanensis had different sensitivities to four antibiotics. Porphyra haitanensis was sensitive to chloramphenicol. When chloramphenicol was 50 μg/mL, the thalloid cells grew slowly, did not differentiate but cell division could be observed. When chloramphenicol was 100 μg/mL, lethal effects were observed, and the higher concentration of the antibiotics, the stronger was the lethal effect. When chloramphenicol was 100-250 μg/mL, more than half of female thalloid lumps died in 14 d. The cleavage of female thalloid cells was restrained strongly by the antibiotics of increasing concentration. Porphyra haitanensis had very strong tolerance to Kanamycin. After cultured for 14 d, the control group was normal. The cells were dark green in color and rhizoid appeared on the margin of the thalloid. The cell development of thalloid of the treated groups was fine. The cells were brownish red, and chromatophores were distinct, at the same time, the ceils were splitting and cell wall thickening, which shows that Kanamycin had no inhibiting effect to the development of thalloid cells. Porphyra haitanensis had certain tolerance to tetracycline. But tetracycline could restrain cleavage and differentiation of thallo

关 键 词：坛紫菜 叶状体 抗生素 敏感性 选择标记 

分 类 号：S968.431[农业科学—水产养殖]