胃癌细胞甲硫氨酸依赖性的蛋白质组学研究  

Analyzing the methionine dependency in human gastric cancer cells by proteomic methodologies

作　　者：辛林[1] 曹伟新[2] 陈雪华[2] 刘炳亚[1] 朱正纲[2] 

机构地区：[1]南昌大学第二附属医院胃肠外科,330006 [2]上海交通大学附属瑞金医院消化外科研究所

出　　处：《中华实验外科杂志》2009年第1期14-16,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家重点基础研究规划“973”计划资助项目（2002CB713703）

摘　　要：目的应用蛋白质组学的技术筛选并鉴定与胃癌SGC-7901细胞甲硫氨酸依赖性相关的蛋白质。方法将胃癌细胞株SGC-7901在同型半胱氨酸替代甲硫氨酸的培养液（M^-H^＋）和正常培养液（M^＋H^-）连续培养5d后抽提细胞总蛋白，应用双向电泳与质谱技术筛选并鉴定差异表达的蛋白质，并用Western blot方法进一步验证差异表达的蛋白质。结果筛选并鉴定出10个差异表达的蛋白。这些蛋白质的功能主要涉及信号传导、抗氧化和药物代谢、细胞内蛋白运输等。结论限制甲硫氨酸环境可能通过激活p38激酶信号传导通路、破坏胃癌细胞抗氧化防御机制及药物解毒功能来抑制肿瘤细胞生长。Objective To screen and identify proteins related to methionine dependency in human gastric cancer cell line. Identification of these proteins is useful for understanding the molecule mechanism of methionine dependency in human gastric cancer cell line. Methods SGC-7901 cells cultured in M ^＋ H^-or M^-H＋ medium for 5 days,which was followed by analysis of total cellular protein from cells by a combination of 2-DE and MS. Then the differential expressional levels of partially identified proteins were validated by Western blot analysis. Results The 10 differential proteins between pairs of gastric cancer cells SGC-7901 cultured either in M^＋ H^-medium or M^-H^＋ medium,were identified by MALDI-TOF-MS and database search. Most of these proteins were shown to be involved in signal transduction,antioxidant or drug detoxification function,intracellular protein transport etc. Conclusion It is possible that methionine restriction induced cell cycle arrest and apoptosis by activating p38 mitogen-activated protein kinase and impairing antioxidant defense and detoxification function in gastric cells.

关 键 词：胃癌 甲硫氨酸 蛋白质组 

分 类 号：R735.2[医药卫生—肿瘤]