低表达蛋白HAX1对Hela细胞凋亡的影响  

Effects of down-regulation of HAX1 protein on apoptosis of Hela cells

作　　者：刘韦成[1] 严群[1] 罗学来[1] 李兆明[1] 王桂华[1] 邓豫[1] 李小兰[1] 陶德定[1] 胡俊波[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肿瘤研究所/胃肠外科,武汉430030

出　　处：《中华实验外科杂志》2009年第1期37-38,共2页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（30872472、30800569）;国家“973”计划资助项目（2009CB521802）;教育部新世纪优秀人才计划资助项目（NCET）（NCET-04-0699）

摘　　要：目的观察低表达蛋白HAX1对Hela细胞维持线粒体膜电位及细胞凋亡的影响。方法利用RNA干扰技术抑制Hela蛋白HAX1表达3d后，利用阳离子脂质荧光染料JC-I标记法和流式细胞仪检测并比较实验组（蛋白HAX1低表达组）及对照组细胞线粒体膜电位变化趋势。加入H2O2（2mmol／L）诱导细胞凋亡3h后利用Annexin V-FITC法检测并比较两组细胞凋亡率。结果对照组细胞线粒体膜电位下降百分比均值为9．52％，实验组细胞线粒体膜电位下降百分比均值为21．12％，实验组细胞线粒体膜电位降低比率多于对照组（P〈0．05）。对照组H2O2（2mmol／L）诱导细胞凋亡率均值为21．80％，实验组H2O2（2mmol／L）诱导细胞凋亡率均值为31．73％。实验组细胞凋亡率高于对照组（P〈0.05）。结论低表达蛋白HAX1不仅可以降低Hela细胞线粒体膜电位稳定性，而且促进Hela凋亡。Objective To investigate the effect of the down-regulation of HAX1 protein on maintenance of mitochondria membrane potential and apoptosis of Hela cells. Methods After 3 days of inhibiting the expression of HAX1 protein by using RNAi technology, the changes in mitochondria membrane potential of Hela cells were observed and compared between experimental group and control group by JC-I labeling method and flow cytometry. Three h after addition of H2 O2 （2 mmol/L） to induce apoptosis, the apoptosis rate was detected by using Annexin V-FITC technique. Results The decreasing percent of cellular mitochondria membrane potential in control and experimental groups was 9.52% and 21.12% in average respectively （P 〈 0.05 ）. The H2 O2 （2 mmol/L） -induced apoptosis rate in control and experimental groups was 21.80% and 31.73% in average respectively （ P 〈 0.05 ）. Conclusion Down-regulation of HAX1 protein can not only reduce the stability of mitochondria membrane potential, but also induce apoptosis of Hela cells.

关 键 词：RNA干扰 线粒体 膜电位 脱噬作用 

分 类 号：R73-3[医药卫生—肿瘤]