C02气腹下缺氧诱导因子-1α与血管内皮生长因子在大鼠肝癌中的表达及其意义  

Expression and significance of HIF-1α and VEGF in liver cancer model of rats under CO2 pneumoperitoneum

作　　者：孙铁为 吕瑞光[1] 孙世波[1] 

机构地区：[1]哈尔滨医科大学附属第二医院普外科,150086

出　　处：《中华实验外科杂志》2009年第1期50-51,共2页Chinese Journal of Experimental Surgery

摘　　要：目的观察不同CO2气腹压力条件下，大鼠移植性肝癌中缺氧诱导因子（HIF）-1α与血管内皮生长因子（VEGF）表达的变化。方法制作大鼠Walker-256移植性肝癌模型，将48只肝癌模型随机分为4组，给予不同气腹压力0、5、10、15mm Hg（1mm Hg=0．133kPa），恒定一定时间及流量。术后处死全部大鼠，切取肝肿瘤，4％甲醛固定后行苏木素-伊红（HE）染色，免疫组织化学染色检测HIF-1d及VEGF表达。结果随着CO2气腹压力的增加，HIF-1α、VEGF在肿瘤模型上的表达逐渐增强（P〈0．05），各组肝癌组织中HIF-1α VEGF呈正相关关系（其r分别为0．835、0．714、0．761、0．781）。结论随着CO2气腹压力的增加，HIF-1α与VEGF的表达逐渐增加，且HIF-1α与VEGF呈正相关。CO2气腹促进肿瘤生长和转移可能与HIF-1α VEGF有一定关系。Objective To observes the influence of CO2 pneumoperitoneum with different pressure on the expression of hypoxia-inducible factor 1α （HIF-1α） and vascular endothelial growth factor （VEGF） in transplanted liver cancer model of rats. Methods Transplanted liver cancer model of rats with Walker-256 was established. Forty-eight liver cancer models were randomly divided into 4 groups of 0,5,10 ,and 15 mmHg pneumoperitoneum pressure （The time and flow rate were permanent）. All rats after operation were killed, and the liver tumors were removed, fixed with 4% formaldehyde, and stained with HE. By using immunohistochemistry staining, the expression of HIF-1α and VEGF was detected. Results With the increased of CO2 pneumoperitoneum pressure, the expression of HIF-1α and VEGF was increased gradually on the tumor model （P 〈 0.05）. There was a positive correlation between HIF-1α and VEGF in liver cancer tissues of each group （r = 0. 835, 0. 714, 0. 761,0.781, respectively）. Conclusion Promo- tion of tumor growth and metastasis by CO2 pneumoperitoneum has certain relation with HIF-1α and VEGF. There is a positive correlation between HIF-1α and VEGF.

关 键 词：癌 肝细胞 C02 气腹 HIF一1 VEGF 

分 类 号：R735.7[医药卫生—肿瘤]