前列腺干细胞抗原在膀胱移行细胞癌中的表达及其临床意义  

Expression and clinical significance of prostate stem cell antigen in the transitional cell carcinoma of bladder

作　　者：余伟民[1] 程帆[1] 张孝斌[1] 阮远[1] 

机构地区：[1]武汉大学人民医院泌尿外科,430060

出　　处：《中华实验外科杂志》2009年第1期92-93,共2页Chinese Journal of Experimental Surgery

基　　金：基会项目：湖北省科技攻关资助项目（2007AA402C22）

摘　　要：目的采用量子点荧光标记技术观察前列腺干细胞抗原在膀胱移行细胞癌中的表达。方法52例膀胱移行细胞癌手术标本，其中病检提示为表浅膀胱癌34例，侵犯肌层的有18例，所有标本行量子点荧光标记，每张设置阴性对照，实验后1个月内，在4个时间点对阳性部位荧光强度分4个等级做出判断。结果在肿瘤细胞膜上出现一圈橙红色的荧光，细胞质和细胞核无荧光表达，阴性片表现为组织自身激发的绿色荧光，两者对照区别明显。这种阳性表达在表浅膀胱肿瘤中达到97．1％，在侵犯肌层的肿瘤中为77．8％，在病理级别为Ⅱ级的肿瘤中强阳性表达率为43．3％，Ⅲ级的强阳性表达率为59．1％。荧光强度在2周内无明显变化，到1个月时标本荧光强度有不同程度减弱，没有了荧光强度为“＋＋＋”的标本，除了4例标本荧光强度衰减为“0”外，其余仍在肉眼可识别范围内。结论前列腺干细胞抗原是膀胱移行细胞癌有价值的瘤标，特别是对高级别表浅肿瘤敏感性高，可用于临床早期诊治和监测复发。Objective Using quantum-dot-fluorescence labeling for detecting the characteristic of prostate stem cell antigen （PSCA） protein expressed in the transitional cell carcinoma （TCC） of bladder. Methods Fifty-two cases of TCC contained 34 superficial TCC （ STCC, stages Ta-T1 ） and 18 muscle-invasive TCCs （ITCC, stages T2-T4）. Quantum-dot-fluorescence labeling analysis of PSCA protein expression was performed on the entire specimens and each of the cases set up a negative control group. The expression of PSCA protein was observed at 4 time points within 1 month, and the level was scored in 4 grades by assessing the intensity of fluorescence. Results The surface of tumor cells express orange red fluorescence under the stimulating of blue light ,the cytoplasm and nucleus with no expression. With obviously control, the negative controls express spontaneous green fluorescence. The expression of fluorescence in STCC was more uniform than that seen in ITCC,with fluorescence detected 97.1% （33/34） ;however, the ITCC also detected 77.8% （14/18）. Higher level of fluorescence expression correlated with the increasing tumor grade. The strong fluorescence expression in grade Ⅱ was 43.3% and 59.1% in grade Ⅲ. The level of the fluorescence change was not significant in 2 weeks, in comparing, the fluorescence hardly keep stabilization last for 1 month, there was 4 specimens with the fluorescence drop to 0 and none 3 ＋ level specimen exist. However, the last specimens distributed in the ＋ and 2 ＋ by fluorescence level could still be seen. Conclusion PSCA is a valuable tumor marker, particularly superficial with higher grade tumor,which prompts that it may be a useful target for early diagnosis and monitor recurrence of TCC of bladder.

关 键 词：膀胱癌 前列腺 干细胞 抗原 

分 类 号：R737.14[医药卫生—肿瘤]