乳酸的液态酶荧光毛细分析法研究  

Research on determination of lactic acid based on liquid-enzyme fluorescence capillary analysis

作　　者：鞠香[1] 高秀峰[2] 李永生[1] 杨微[1] 

机构地区：[1]四川大学化学工程学院,成都610065 [2]四川大学华西基础与法医学院,成都610041

出　　处：《分析试验室》2009年第1期120-122,共3页Chinese Journal of Analysis Laboratory

基　　金：四川大学214振兴计划基金(0082204127098)项目资助

摘　　要：基于乳酸脱氢酶(LDH)和烟酰胺腺嘌呤二核苷酸(NADH)氧化还原体系,提出了乳酸的液态酶荧光毛细分析方法(LE-FCA)。在激发波长350 nm、发射波长460 nm条件下,用LE-FCA法对乳酸进行了测定;其线性范围为0.2～1.0 mmol/L,r>0.9932,检出限为0.022 mmol/L,RSD<4.2%。LE-FCA能节省贵重的酶试剂,样品用量仅为18μL;可用于医药、卫生、工业、食品等含乳酸样品的测定。A new method for the determination of lactic acid the enzymatic fluorescence capillary analysis has been proposed. A capillary is used as a carder for enzyme and lactic acid determination in the study. The optimum experimental conditions are as follows： the reaction time is 15 min, the temperature is 38 ℃, the concentrations of Tris-HCl buffer solution （pH 8.80） and NAD^＋ are 0.1 mmol/L and 3.0 mmol/L, respectively; the concentration of LDH is 400 U/L. The linear range for the determination of lactic acid is 0.2 - 1.0 mmol/L with a correlation coefficient of r 〉 0.9932, the detection limit is 0.022 mmol/L, and RSD is less than 4.2%. This method has a better reproducibility, and simpler operation. The total volume used in the test is only 18μL. It is help full for saving the expensive enzyme reagent and decreasing the test cost. The LE-FCA method can be applied to the determination of lactic acid in multi components and in various fields of fermentable food, leechdom, blood samples, industrial reagents, etc.

关 键 词：乳酸 荧光毛细分析 液态酶 烟酰胺腺嘌呤二核苷酸 

分 类 号：O657.8[理学—分析化学]