茶多酚与抗肿瘤药物联合作用对人鼻咽癌耐药细胞株HNE-1增殖抑制的影响  

Inhibitory Effect of Tea Polyphenols in Combination with Anti-tumor Drugs on Cell Proliferation of the Multiresistant HNE-1 Cell Line

作　　者：王驰[1] 陈鸿雁[2] 叶琳[2] 沈娜[2] 张潜英[2] 余晓燕[2] 

机构地区：[1]重庆医科大学药学院,重庆市400016 [2]重庆医科大学附属第一医院耳鼻喉科,重庆市400016

出　　处：《中国药房》2009年第1期33-35,共3页China Pharmacy

基　　金：重庆市卫生局中医处资助课题(渝中医[2006]27号2006-B-16)

摘　　要：目的:检测茶多酚与4种抗肿瘤药物长春新碱、平阳霉素、5-氟尿嘧啶(5-Fu)、顺铂联合应用对放射线照射后的鼻咽癌耐药细胞株HNE-1(200)的增殖抑制作用。方法:采用MTT法筛选茶多酚及4种药物的低毒剂量,再测定低毒剂量的茶多酚与4种药物联合应用后对HNE-1(200)细胞的增殖抑制率。结果:茶多酚的低毒剂量为0.50mg.mL-1,该剂量下茶多酚与长春新碱、平阳霉素、5-Fu、顺铂联合应用后,对HNE-1(200)细胞的增殖抑制率均不同程度的增加,其增幅为单用抗肿瘤药的100%～300%。结论:茶多酚与4种抗肿瘤药物低剂量联合应用对人鼻咽癌耐药细胞具有明显的增殖抑制作用,提示茶多酚对该细胞株具有一定的耐药逆转作用。OBJECTIVE： To study the joint action of tea polyphenols and common anti - tumor drugs （vincristine, pingyangmycin, 5 Fu, cisplatin） in inhibiting cell proliferation of the multiresistant HNE 1 （200） cell line in patients with nasopharyngeal carcinoma after radiographic exposure. METHODS： The low toxic dosage of tea polyphenols and 4 kinds of anti - tumor drugs were optimized by MTT method. Then the inhibition ratio of multiresistant HNE - 1（200） cell line treated by tea polyphenols and anti - tumor drugs waere detected. RESULTS： The low toxic dosage of tea polyphenols was 0.50 mg · mL^-1. When the anti - tumor drugs were used in combination with tea polyphenols （0.50 mg · mL^-1） as compared without, the inhibition ratio on HNE - 1（200） cell line was increased by 100% -300%. CONCLUSIONS： Addition of tea polyphenols to anti-tumor drugs showed remarkable inhibitory effect on cell proliferation of HNE- 1 （200） cell line in patients with nasopharyngeal carcinoma, suggesting the reverse effect of tea polyphenols on resistant HNE - 1（200） cell line.

关 键 词：茶多酚 抗肿瘤药物 鼻咽癌耐药细胞株 增殖抑制 

分 类 号：R965[医药卫生—药理学] R979.1[医药卫生—药学]