胎盘型谷胱甘肽S-转移酶在人肝癌及癌旁组织的表达  

EXPRESSION OF PLACENTAL GLUTATHIONE S TRANSFERASE IN HEPATOCELLULAR CARCINOMA AND ITS SURROUNDING TISSUES

作　　者：邹伟[1] 张昭成[1] 李慎谦[1] 牛兆山[1] 李玉军[1] 

机构地区：[1]青岛医学院

出　　处：《青岛医学院学报》1998年第1期8-9,共2页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省教委科研基金

摘　　要：①目的探讨胎盘型谷胱甘肽Ｓ－转移酶（ＧＳＴπ）在肝癌及癌旁组织的表达及意义。②方法应用抗人ＧＳＴπ多克隆抗体，以ＬＳＡＢ免疫组织化学方法检测７７例肝细胞癌及癌旁组织中ＧＳＴπ的表达。③结果７７例肝细胞癌中，ＧＳＴπ阳性表达率为８９．６％；癌旁组织中，增生结节ＧＳＴπ阳性表达率为７１．１％（２７／３８），肝硬化组织为２２．９％（８／３５）；肝癌及增生结节中ＧＳＴπ阳性表达率显著高于肝硬化组织（χ２＝４４．９，１６．９；Ｐ＜０．００１）；且肝癌与增生结节两组间ＧＳＴπ阳性表达率亦有显著性差异（χ２＝６．３５，Ｐ＜０．０２）。ＧＳＴπ表达与Ｅｄｍｏｎｄｓｏｎ组织分级无关（χ２＝１．９６，Ｐ＞０．２５）。④结论ＧＳＴπ可作为肝细胞癌及癌前病变的标志酶。Objective To study the value of expression of glutathione S transferase placental form (GSTπ) in human hepatocellular carcinoma(HCC) and its surrounding tissues. Methods GSTπ was detected in 77 cases of HCC and their surrounding tissues with LSAB immunohistochemical technique. Results Positive expression rate of GSTπ was 89.7% in HCC;In pericarcinomatous tissues, GSTπ positive rate in hyperplastic nodules and in cirrhosis was 71.1% and 22.9% respectively; The positive rate of GSTπ in HCC and hyperplastic nodule was significantly higher than that in cirrhosis( χ 2=44.9,16.9;P< 0.001); The GSTπ positive rate in HCC was higher than that in hyperplastic nodules( χ 2=6.35,P< 0.02). GSTπ expression was not correlated with HCC Edmondson's grade( χ 2=1.96,P> 0.25). Conclusion GSTπ is a new marker for immunohistochemical detection of human HCC and its preneoplastic lesions, and it may be valuable in the early diagnosis of HCC.

关 键 词：肝肿瘤 谷胱甘肽 转移酶 GSTΠ 癌前病变 

分 类 号：R735.702[医药卫生—肿瘤] R730.4[医药卫生—临床医学]