液相色谱-质谱/质谱法快速测定保健品中违禁成分麻黄碱、伪麻黄碱和盐酸芬氟拉明  

Rapid Detection of Ephedrine、Pseudoephedrine and Fenfluramine Hydrochloride in Health Products by High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：郭俊峰 杨冀州 李淑娟[2] 

机构地区：[1]河南出入境检验检疫局技术中心,郑州450003 [2]中国检验检疫科学研究院,北京100025

出　　处：《中国国境卫生检疫杂志》2008年第6期415-417,429,共4页Chinese Journal of Frontier Health and Quarantine

基　　金：国家标准制定项目(20051199-T-424)

摘　　要：〔目的〕建立快速测定保健品中违禁添加成分麻黄碱、伪麻黄碱和盐酸芬氟拉明的液相色谱-质谱/质谱（LC-MS/MS）检测方法。〔方法〕样品用甲醇-水（8＋2）提取后,采用LC-MS/MS电喷雾正离子（ESI＋）电离,多反应监测（MRM）模式检测,外标法定量。〔结果〕麻黄碱、伪麻黄碱和盐酸芬氟拉明的方法检测限分别为0.5mg/kg、0.5mg/kg、0.05mg/kg。在添加水平为0.5～25mg/kg的范围内,麻黄碱和伪麻黄碱的回收率为82.0%～99.2%;相对标准偏差（RSD）不高于7.9%;在添加水平为0.05～2.5mg/kg的范围内,盐酸芬氟拉明的回收率为83.1%～99.6%;相对标准偏差（RSD）不高于6.8%。〔结论〕本方法准确、灵敏、快捷,能够满足对保健品中违法添加的麻黄碱、伪麻黄碱和盐酸芬氟拉明的检测要求。Objective An effective rapid method was developed to determine ephedrine.pseudoephedrine and fenfluramine hydrochloride in health products by high performance liquid chromatography-tandem mass spectrometry （LC-MS/MS）. Method Samples were extracted with methanol-water （8＋2）, Electro-spray ionization in positive mode （ESI＋） and muhiple reaction monitoring （MRM） were adopted, and external standard method was conducted. Result Limits of detection for ephedrine ,pseudoephedrine and fenfluramine hydrochloride were 0.5 mg/kg,0.5 mg/kg ,0.05 mg/kg respectively .The recovery rate of ephedrine and pscudoephedrine was 82.0%-99.2% in the range of 0.5-25 mg/kg.The RSD was less than 7.9%. The recovery rate of fenfluraminc hydrochloride was 83.1% -99.6% in the range of 0.05-2.5 mg/kg.The RSD was less than 6.8%. Conclusion This method is accurate, sensitive, and rapid, h can good meet demand on rapid detection of Ephedrine, Pseudoephedrine and Fenflurarmine hydroehloride in health products.

关 键 词：保健品 麻黄碱 伪麻黄碱 盐酸芬氟拉明 色谱法 液相 质谱法 电喷雾 

分 类 号：R155.5[医药卫生—营养与食品卫生学]