MAGE-A1、MAGE-C2及SSX-1在原发性肝细胞癌中的表达及意义  

The expression of cancer-testis antigen MAGE-A1、MAGE-C2 and SSX-1 mRNA in hepatocellular carcinoma

作　　者：蔡俊[1] 张富喜[1] 陈红松[2] 

机构地区：[1]北华大学附属医院,吉林吉林132011 [2]北京大学人民医院

出　　处：《山东医药》2008年第45期1-3,共3页Shandong Medical Journal

基　　金：教育部2007年度"新世纪优秀人才支持计划"项目(985-2-099-113);863项目(2007AA021103)

摘　　要：目的检测肿瘤—睾丸抗原(CT抗原)MAGE-A1、MAGE-C2和SSX-1 mRNA在原发性肝细胞癌(HCC)中的表达情况。方法采用RT-PCR方法检测MAGE-A1、MAGE-C2和SSX-1 mRNA在55例HCC患者癌及癌旁组织中的表达,对产物进行测序,并对检测结果与临床指标进行分析。结果55例HCC患者的癌组织中,MAGE-A1、MAGE-C2和SSX-1 mRNA的表达率分别为60.0%、14.5%和40.0%;癌旁组织无表达。结论CT抗原MAGE-A1、MAGE-C2和SSX-1 mRNA在HCC中呈高表达,是HCC免疫治疗的理想靶位。Objective To investigate the expression of MAGE-A1, MAGE-C2 and SSX-1 mRNA in hepatocellular carcinoma. Methods Reverse-transcription polymerase chain reaction （RT-PCR） was used to investigate the mRNA expression of the three cancer-testis antigens （ MAGE-A1, MAGE-C2 and SSX-1 ） in patients with HCC. PCR products of cancer-testis antigens were sequenced. The correlation between the expression of cancer-testis antigen and clinical parameters was analyzed. Results In 55 patients with HCC, the rate of MAGE-A1, MAGE-C2 and SSX-1 mRNA expression was 60.0%, 14.5% and 40.0%, respectively. However, mRNAs of these CT antigen were not detected in the adjacent noncancerous tissues. Conclusion MAGE-A1, MAGE-C2 and SSX-1 mRNAs are expressed with high percentages in HCC, indicating that they may be candidates for antigen-specific immunotherapy targets in HCC.

关 键 词：肿瘤—睾丸抗原 癌 肝细胞 逆转录—聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤]