难治性癫痫患者血清和脑脊液中MCP-1表达的研究  

The expression of MCP-1 in serum and cerebrospinal fluid of patients with intractable epilepsy

作　　者：唐玫[1] 吴原[1] 王学峰[2] 庞家容[1] 冯云[1] 刘秀颖[1] 李家荃[3] 

机构地区：[1]广西医科大学第一附属医院神经内科,广西南宁530021 [2]重庆医科大学一附院神经内科,重庆400016 [3]广西医科大学科学实验中心,广西南宁530021

出　　处：《中风与神经疾病杂志》2008年第6期673-676,共4页Journal of Apoplexy and Nervous Diseases

基　　金：广西卫生厅课题(桂卫:Z2006047)

摘　　要：目的研究单核细胞趋化蛋白-1(monocyte chemottractant protein-1 MCP-1)在难治性癫痫患者血清和脑脊液中的表达。方法采用RT-PCR和ELISA技术测定难治性癫痫患者、非难治性癫痫患者、正常对照组血清及/或脑脊液中MCP-1 mRNA及蛋白的含量。结果难治性癫痫患者血清及脑脊液中的MCP-1 mRNA及蛋白的含量较非难治性癫痫明显升高(P<0.01),血清中含量较正常对照组明显增高(P<0.01);非难治性癫痫患者血清中MCP-1 mRNA及蛋白的含量与正常对照组比较差别无统计学意义(P>0.05)。结论MCP-1在难治性癫痫患者血清及脑脊液中均升高,MCP-1可能参与了难治性癫痫的发病过程。Objective To study the expression of MCP-1 in serum and cerebrospinal fluid（CSF） of patients with intractable epilepsy. Methods The mRNA and protein expressions of MCP-1 in serum and/or CSF of patients with intractable epilepsy,non-intractable epilepsy and normal control group were studied by RT-PCR and ELISA. Results The mRNA and the protein expressions of MCP-1 in serum and CSF intractable epilepsy group were significantly higher than non-intractable epilepsy group（P 〈0. 01 ） ,and those expressions were greatly higher than control group in serum（P 〈0. 01 ） ,and the expressions of MCP-1 in non-intractable epilepsy group were not higher than those in control group, the difference had no statistical significance（P 〉 0. 05 ）. Conclusion MCP-1 significantly increased in serum and CSF of the patients with intractable epilepsy. The increases expression of MCP-1 may play important role in the process of intractable epilepsy.

关 键 词：难治性癫痫 单核细胞趋化因子-1 血清 脑脊液 

分 类 号：R742.1[医药卫生—神经病学与精神病学]