转化生长因子β1对缺血再灌注损伤肺组织肿瘤坏死因子α及白细胞介素1β含量的调节  

Regulation of transforming growth factor-beta 1 on expression of tumor necrosis factor alpha and interleukin 1 beta in rats following lung ischemia-reperfusion injury

作　　者：朱震[1] 王志农[1] 

机构地区：[1]解放军第二军医大学附属长海医院胸外科,上海市200433

出　　处：《中国组织工程研究与临床康复》2008年第50期9862-9865,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:肺缺血再灌注时,大量聚集在肺内的白细胞被激活,释放多种细胞因子如肿瘤坏死因子α、白细胞介素1β等,进而介导再灌注损伤。新近研究发现,转化生长因子β1对犬移植肺组织缺血再灌注损伤有保护作用。目的:观察转化生长因子β1干预后,肺缺血再灌注损伤肺组织肿瘤坏死因子α和白细胞介素1β的含量变化。设计、时间及地点:随机对照动物实验,于2007-08/10在解放军第二军医大学附属长海医院胸心外科实验室完成。材料:清洁级SD大鼠30只,随机分为假手术组、缺血再灌注组、转化生长因子β1组,10只/组。转化生长因子β1为PeprotechINC.公司产品。方法:缺血再灌注组与转化生长因子β1组大鼠通过结扎肺门阻断肺循环1h、再灌注2h建立原位肺缺血再灌注损伤模型,假手术组大鼠仅以10#丝线绕过肺门后不结扎。肺循环阻断前15min,各组颈静脉注射肝素抗凝,转化生长因子β1组同时注射10μg/kg转化生长因子β1。主要观察指标:再灌注2h后摘取左肺组织,光镜下观察肺组织形态变化,测定湿干重比及肺泡损伤指数,ELISA法检测肺组织匀浆中肿瘤坏死因子α和白细胞介素1β的含量。结果:30只大鼠均进入结果分析。假手术组肺组织无明显病理改变;缺血再灌注组可见明显的组织水肿及中性粒细胞浸润;转化生长因子β1组轻微组织水肿,少量中性粒细胞浸润。与假手术组比较,缺血再灌注组湿干重比及肺泡损伤指数均显著升高(P<0.01);与缺血再灌注组比较,转化生长因子β1组上述两项指标均明显降低(P<0.05)。与假手术组比较,缺血再灌注组肿瘤坏死因子α及白细胞介素1β含量均显著升高(P<0.01);与缺血再灌注组比较,转化生长因子β1组上述两项指标均明显降低(P<0.05,P<0.01)。结论:转化生长因子β1能够明显改善肺缺血再灌注损伤,该作用与其有效抑制肿瘤坏死因子α及白细胞介素1β的表BACKGROUND： When leucocytes were activated in the process of lung ischemia-reperfusion, various cell factors will be released, including tumor necrosis factor α （TNF-α ） and interleukin 1β （1L-Iβ ）, which lead to ischemia-reperfusion injury. Recent research demonstrates that transforming growth factor- β 1 plays a protective role in ischemia-reperfusion injury after lung transplantation OBJECTIVE： To investigate the level changes of TNF-α and IL-1β after interfered with transforming growth factor- β 1 after lung ischemic-reperfusion injury DESIGN, TIME AND SETTING： The randomized controlled experiment of animal was performed in the Cardiothoracic Surgery Laboratory of Changhai Hospital, Second Military Medical University of Chinese PLA from August to October 2007. MATERIALS： Thirty Sprague-Dawley rats with clean grade, were randomly divided into the sham operation, ischemia-reperfusion and transforming growth factor β1 groups, with 10 animal in each group. The transforming growth factor β1 was phuchased from Peprotech INC. METHODS： The left lung of rat in the ischemia-reperfusion and transforming growth factor β1 groups was prepared for to ischemia-reperfusion model by blocking pulmonary circulation with hilar vessels ligation for 60 minutes, followed by reperfusion for 2 hours. Animals in the sham operation group were crossed hilar with 10# silk thread. Heparin was administered intravenously through vena cervicalis at 15 minutes before ischemia in each group. Concurrently, 10μg/kg transforming growth factor β1 was administered in transforming growth factor β1 group. MAIN OUTCOME MEASURES： After 2 hours of ischemia-reperfusion, the tissues of left lung were removed, histomorphological changes, wet-dry weight ratio, the index of histological lung injury and the content of TNF- α and IL-1β were determined by light microscope, enzyme-linked immunosorbent assay （ELISA）, respectively. RESULTS： A total of 30 animals were enrolled in the result analysis. There was no obvious pat

关 键 词：转化生长因子Β1 肿瘤坏死因子Α 白细胞介素1Β 肺缺血再灌注 

分 类 号：R000[医药卫生]