胰腺癌多细胞团簇的化疗药物敏感试验  

Chemosensitivity of pancreatic cancer ceil in multicellular spheroids

作　　者：周涛[1] 胡亚[1] 廖泉[1] 赵玉沛[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院基本外科,北京100730

出　　处：《中华胰腺病杂志》2008年第6期361-364,共4页Chinese Journal of Pancreatology

基　　金：国家自然科学基金（30772526）

摘　　要：目的探讨应用胰腺癌多细胞团簇做化疗药物敏感试验的可行性。方法应用胶原凝胶微滴三维培养胰腺癌细胞株SWl990、PCT-3和ASPC-1细胞。在形成多细胞团簇后，采用CD-DST法和CCK-8法测定其对不同浓度的5-氟尿嘧啶（5-FU）、健择（GEM）及奥沙利铂（OXA）3种化疗药物的敏感性，并与分散细胞模型进行比较。结果形成多细胞团簇的3种胰腺癌细胞对不同浓度5-FU、GEM及OXA三种药物的敏感性均较分散细胞的敏感性显著下降（P〈0．05）。50μg/m15-FU对SW1990、PCT．3、ASPC-1多细胞团簇的抑制率分别为（53．96±4．32）％、（58．49±5．98）％、（49．57±4．36）％；25μmlGEM对SWl990、PCT-3、ASPC-1细胞团簇的抑制率为（53．02±4．06）％、（61．90±4．89）％、（38．09±4．88）％，10μg／mlOXA对3种细胞团簇的抑制率为（57．33±6．27）％、（50．90±4．90）％、（47．26±4．29）％，均显著低于对分散细胞的抑制率（P〈0．05）。结论肿瘤细胞形成细胞团簇后对抗肿瘤药物的敏感性明显降低，耐药性增加，更符合体内状态。Objective To investigate the feasibility of pancreatic cancer multicellular spheroids in chemosensitivity study. Methods Pancreatic adenocarcinoma cell lines SW1990, PCT-3 and ASPC-1 were cultured in collagen gel. When the muhicellular spheroids were formed, the drug chemosensitivities of Fluorouraeil （5-FU）, Gemzar （GEM） and Oxaliplatin （OXA） were detected by CD-DST and CCK-8 methods. The results were compared with those of scattered cells model. Results The chemosensitivities of three pancreatic lines in form of muhicellular spheroids were significantly lower than those of scattered cells model （P 〈 0.05）. The inhibitory rates of 50 μg/ml 5-FU on SW1990, PCT-3, ASPC-1 were （53.96 ± 4.32 ）%, （58.49 ± 5.98 ） %, （49.57 ± 4.36 ） % ; the inhibitory rates of 25 μg/ml GEM on SW1990, PCT-3, ASPC-1 were （53.02 ± 4.06） % , （61.90 ± 4.80） % , （38.09 ± 4.88） % ; the inhibitory rates of l0 μg/ml OXA on SW1990, PCT-3, ASPC-1 were （57.33 ± 6.27 ） %, （ 50.90 ± 4.80） %, （47.26 ± 4.29 ） % ; which were significantly lower than those of scattered cells model （ P 〈 0.05 ）. Conclusions In collagen gel, formation of the muhicellular spheroids of pancreatic cells caused less chemosensitivity and promoted anticancer drug resistance, which was consistent with in vivo state.

关 键 词：胰腺肿瘤 球形体 细胞 药物筛选试验 

分 类 号：R735.9[医药卫生—肿瘤]