雷公藤内酯醇抑制胰腺癌细胞生长及血管生成的实验研究  

Inhibitory effect of Tripotolide on the growth of pancreatic cancer cell line and transplanted tumor and angiogenesis

作　　者：周国雄[1] 丁晓凌[1] 张弘[1] 魏群[1] 吴深宝[1] 成建萍[1] 强晖[1] 黄介飞[1] 

机构地区：[1]南通大学附属医院消化内科,江苏南通226001

出　　处：《中华胰腺病杂志》2008年第6期405-407,共3页Chinese Journal of Pancreatology

基　　金：江苏省自然科学基金（BK2004049）

摘　　要：目的探讨雷公藤内酯醇（TL）对胰腺癌细胞生长及肿瘤新生血管生成的抑制作用。方法采用CCK-8试剂盒检测TL对胰腺癌SW1990细胞的生长抑制作用；采用TUNEL法检测TL对细胞凋亡的诱导作用；观察尾静脉注射TL对裸鼠移植瘤生长抑制作用和对瘤组织VEGF表达、微血管密度（MVD）的影响。结果TL呈浓度和时间依赖性抑制SW1990细胞增殖，160mg／ml的TL干预SW1990细胞24h，细胞抑制率达50．6％，细胞凋亡率从对照组的9．6％升高到45．1％（P〈0．01）；TL0．5mg／kg体重瘤内注射对移植瘤的抑瘤率达89．9％，瘤组织VEGF表达减少，MVD从36．25±8．64减少到9．87±3．34（P〈0．01）。结论 TL可显著抑制胰腺癌细胞增殖并诱导细胞凋亡；通过抑制肿瘤新生血管生成可抑制裸鼠SW1990细胞移植瘤的生长。Objective To investigate the suppression effects of Tripotolide （TL） on the pancreatic cancer xenograft models and angiogenesis. Methods The growth suppression effect of TL on SW1990 was determined using cell count kit （CCK-8）, apoptotic cells induced by TL were examined by morphology and terminal deoxynucleotidyl transferase-mediated nick end labeling （TUNEL） assay. The inhibitory effects of TL on the growth of tumor xenografts and tumor microvascular density （MVD） were investigated. Results TL inhibited the growth and proliferation of SW1990 cells in a concentration-dependent and time-dependent manner. The inhibition ratios of cells treated at 160 mg/ml TL for 24 h was 50.6%, the apoptotic rate increased from 9.6% in the control group to 45.1% （P 〈 0.01 ）. The inhibition rate of cancer xenograft growth was 89.9% when TL was intratumorally injected at the dose of 0.5 mg/kg. The expression of VEGF in tumor tissue decreased while MVD also decreased from 36. 25 ± 8. 64 to 9. 87 ± 3. 34 （P〈0.01）. Conclusions TL induced prominent growth inhibition and apoptosis in human pancreatic cancer cell lines. TL can attenuate the growth of pancreatic caner xenografts through its effect on antiangiogenesis.

关 键 词：胰腺肿瘤 雷公藤内酯 移植瘤 血管内皮生长因子 

分 类 号：R735.9[医药卫生—肿瘤]