乙型肝炎病毒基本核心启动子突变与基因型的关系  

Relationship between hepatitis B virus basic core promoter mutations and viral genotypes

作　　者：路文明[1] 童福易[1] 曹文贵[1] 吴建鸿[1] 吴越人[1] 

机构地区：[1]苏州市第五人民医院传染病科,215007

出　　处：《肝脏》2008年第6期472-474,共3页Chinese Hepatology

摘　　要：目的研究乙型肝炎病毒(HBV)基本核心启动子(BCP)突变与HBV基因型的关系。方法随机选取我院68例慢性乙型肝炎患者外周血,采用荧光定量PCR结合TaqmanMGB探针技术检测HBV基因型,并用基因扩增和DNA测序方法检测BCPT1762/A1764双突变。结果68例患者HBV分型中,B基因型20例,C基因型46例,B、C混合型1例,未分型(非B非C型)1例。66例B、C两基因型中,B基因型组T1762/A1764双突变5例,突变率25.0%(5/20),C基因型组T1762/A1764双突变24例,突变率52.2%(24/46),C基因型T1762/A1764双突变率明显高于B基因型(P<0.05)。结论苏州地区慢性乙型肝炎患者基因型以C型和B型为主,C基因型比B基因型更易发生T1762/A1764双突变。Objective To investigate the relationship between hepatitis B virus（HBV） basic core promoter （BCP） mutations and viral genotypes. Methods Sera from 67 patients chronically infected with HBV were tested. HBV genotype was determined by real-time polymerase chain reaction （PCR） using TaqMan-MGB probe technology. Doublemutation in BCP（T1762/A1764） were determined by PCR assay and direct DNA sequeneing. Results The doublemutation in BCP（T1762PA1764） was significantly more frequent in genotype C patients than that in genotype B patients （52.2 % vs 25.0 % ,X^2 = 4. 179 , P〈0.05）. Conclusion Genotype C and genotype B are the two dominant genotypes in patients living in Suzhou area. Compared with genotype B , the double-mutation in BCP（T1762/A1764） was more common in patients with genotype C patients.

关 键 词：乙型肝炎病毒 基本核心启动子 突变 基因型 

分 类 号：R512.62[医药卫生—内科学] R450[医药卫生—临床医学]