超声联合SonoVue介导荧光蛋白质粒转染人胚肾293T细胞的研究  

Gene transfer with pEGFP into human embryo kidney cell 293T mediated by ultrasound and SonoVue

作　　者：郑颖[1] 王文平[1] 赵平[2] 王宏卫[2] 赵奕文[3] 

机构地区：[1]复旦大学附属中山医院超声科,上海200032 [2]第二军医大学微生物教研室,上海200433 [3]上海市徐汇区中心医院超声室,上海200030

出　　处：《同济大学学报（医学版）》2008年第6期28-31,共4页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金资助项目(30500606)

摘　　要：目的探讨超声协同微泡造影剂SonoVue介导荧光蛋白质粒转染人胚肾293T细胞的方法。方法以不同的超声辐照时间、超声机械指数和不同浓度的微泡造影剂SonoVue的参数组合,将含有绿色荧光蛋白报告基因的真核表达质粒pEGFP-N1转染人胚肾293T细胞,接种于6孔板中,以荧光显微镜观察观察293T细胞中的绿色荧光蛋白表达情况并用流式细胞仪测算转染率。结果当微泡造影剂SonoVue浓度为40%、超声机械指数为1.0、辐照时间5 min时,质粒的转染率最高(28.11±1.63)%。结论超声联合微泡造影剂SonoVue能够作为载体介导质粒转染细胞,但需优化各项超声辐照的条件以达到最佳效果。Objective To explore a method to transfer plasmid of enhanced green fluorescent protein （pEGFP） into human embryo kidney 293T cell mediated by ultrasound and SonoVue. Methods Under different parameters of ultrasound such as ultrasound exposure time and mechanical index （MI） and different SonoVue dosages, the expression of pEGFP was studied by fluorescence microscope and the transfection efficiency were calculated by flow cytometry. Results 1.0 with 40% SonoVue led to the highest transfection SonoVue could transfer pEGFP into cells. However, When the ultrasound exposure at 5 minutes and MI at effect（28.11 ±1.63）%. Conclusion Ultrasound and more parameters should be coordinated to achieve the best effect.

关 键 词：超声 微泡造影剂 人胚肾细胞293T 基因转染 

分 类 号：R445.1[医药卫生—影像医学与核医学]