豆野螟触角感器的电镜超微结构及嗅觉功能  

Ultrastructure of antennal sensilla of Maruca testulalis (Lepidoptera:Pyralidae) adult and its sensory responses to sex pheromone and plant volatiles

作　　者：王霞[1] 徐静 刘凤英[1] 陈海滨[1] 吴降星 杜永均[1] 

机构地区：[1]温州医学院健康与环境生态研究所,浙江温州325035 [2]温州市农业科学研究院,浙江温州325006 [3]宁波市农业技术推广总站,浙江宁波315012

出　　处：《昆虫学报》2008年第12期1225-1234,共10页Acta Entomologica Sinica

基　　金：浙江省重点项目(2006C22001);温州市重大项目(N2005B030)

摘　　要：本文利用扫描电镜(SEM)和触角电位仪(EAG)对豆野螟Maruca Testulalis(Geyer)触角感器的结构其及嗅觉功能进行了研究。豆野螟雌、雄成虫触角外部形态结构扫描的结果表明,豆野螟雌成虫触角上存在有毛形感器、刺形感器、腔锥形感器、耳形感器和栓锥形感器5种,雄成虫触角除上述5种感器外,还分布有柱形感器。以基部(触角的1/3)、中部(触角的1/3)和端部(触角的1/3)为单位,对雌、雄成虫触角节的各种类型感器数量进行统计,结果表明毛形感器、腔锥形感器、耳形感器在雌和雄虫触角上的分布都存在极其显著的差异。测定了豆野螟雄虫触角对性信息素化合物反,反-10,12-十六碳二烯醛和反,反-10,12-十六碳二烯-1-醇以及雌虫触角对植物挥发性化合物顺-3-己烯-乙酸酯和芳樟醇的EAG反应感应部位,结果表明EAG反应强弱与感器数量之间不存在明显的线性相关;对性信息素和植物挥发物有反应的触角感器在触角上的分布存在差异,对信息素反应的感器主要分布在触角鞭节的顶端24小节内(约占触角的1/3),而对植物挥发性化合物反应的感器则分布于触角鞭节顶端48小节内(约占触角的2/3)。The structure and olfactory function of antennal sensilla of the bean pod borer Maruca testulalis （Geyer） （Lepidoptera： Pyralidae ） were investigated by the scanning electron microscopy （SEM） and electroantennogram （EAG） technology. The results showed that there are five types of sensilla in both male and female antenna, including sensilla trichodea, sensilla chaetica, sensilla eoeloconicum, sensilla auricillica and sensilla styloconicum, whereas another type of sensilla, sensilla cylindric, was observed only in the male antenna. The number of all types of sensilla in both male and female adults was statistically analyzed by dividing the antenna into three sections （basal, middle and distal）. It was found that the quantity of sensilla was significantly different between male and female in three types of sensilla （sensilla trichodea, sensilla coeloconicum and sensilla auricillica）. The EAG responses of male and female adults to sex pheromone, （E10, E12）-hexadecadienal and （E10, E12）-hexadeeadien-1-ol, and plant volatiles, cis-3-hexenyl acetate and （1）-linalool, showed no correlation between the number of sensilla and the EAG amplitude. The sensilla contributed to the sensory responses to pheromone compounds are mainly distributed in 24 distal flagellar segments of the antenna （1/3）, but the segments detecting the plant volatiles are distributed in 48 distal flagellar segments of the antenna （2/3）.

关 键 词：豆野螟 触角感器 扫描电镜 触角电位 植物挥发性化合物 性信息素化合物 

分 类 号：Q964[生物学—昆虫学]