Identification of AOSC-binding proteins in neurons  

作　　者：刘明 聂琴 辛现良 耿美玉 

机构地区：[1]Department of Molecular Pharmacology,School of Medicine and Pharmacy Ocean University of China [2]Division of Anti-tumor Pharmacology,State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2008年第4期394-399,共6页中国海洋湖沼学报（英文版）

基　　金：Supported by National Basic Research Program of China (973 Program) (No.2003CB716400);Natural Science Foundation of China for Distingutshed Young Scholars (No.30725046);Natural Science Foundation of China (No.30472029,No.30772612);Chinese High technology Research and Development Program of China(863 Program) (No.2006AA090501)

摘　　要：Acidic oligosaccharide sugar chain （AOSC）, a D-mannuronic acid oligosaccharide, derived from brownalgaepolysaccharide, has been completed Phase I clinical trial in China as an anti-Alzheimer＇s Disease （AD） drug candidate. The identification of AOSC-binding protein（s） in neurons is very important for understanding its action mechanism. To determine the binding protein（s） of AOSC in neurons mediating its anti-AD activities, confocal microscopy, affinity chromatography, and liquid chromatography-tandem mass spectrometry （LC-MS/MS） analysis were used. Confocal microscopy analysis shows that AOSC binds to SH-SY5Y cells in concentration-, time-, and temperature-dependent fashions. The AOSC binding proteins were purified by affinity chromatography and identified by LC-MS/MS analysis. The results showed that there are 349 proteins binding AOSC, including clathrin, adaptor protein-2 （AP-2） and amyloid precursor protein （APP）. These results suggest that the binding/entrance of AOSC to neurons is probably responsible for anti-AD activities.酸的 oligosaccharide 糖链(AOSC ) ， D-mannuronic 酸 oligosaccharide，源于棕色的水藻多糖，被完成了阶段我在是的中国的临床的试用 anti-Alzheimer 的疾病(广告) 药候选人。在神经原的 AOSC 有约束力的蛋白质的鉴定为理解它的行动机制是很重要的。为了在调停的神经原决定 AOSC 的有约束力的蛋白质，它的反广告活动，共焦的显微镜学，亲密关系层析，和液体层析双人脚踏车集中 spectrometry (LC-MS/MS ) 分析被使用。AOSC 在集中绑在 SH-SY5Y 房间的共焦的显微镜学分析表演 -- ，时间 -- ，并且温度依赖者时尚。AOSC 有约束力的蛋白质被亲密关系层析净化并且由 LC-MS/MS 分析识别了。结果证明有 349 蛋白质绑定 AOSC，包括 clathrin，适配器 protein-2 (AP-2 ) 和淀粉的先锋蛋白质(应用软件) 。这些结果建议到神经原的 AOSC 的绑定 / 入口为反广告活动可能负责。

关 键 词：AOSC Alzheimer＇s disease marine oligosaccharide binding protein（s） 

分 类 号：R741[医药卫生—神经病学与精神病学]