SNCG基因转染对PC-3细胞抗肿瘤药物作用效果的影响  

Influence of SNCG Transfection on the Effectiveness of Anti-tumor Drugs in PC-3 Cell Lines

作　　者：陈家梁[1,2] 王波[1,2] 张丽荣[2] 何帮顺[2] 潘玉琴[2] 曾庆娣[1,2] 蒋华新[2] 王书奎[2] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210046 [2]南京医科大学附属南京第一医院中心实验室,江苏南京210006

出　　处：《中华男科学杂志》2008年第12期1077-1082,共6页National Journal of Andrology

基　　金：南京市科技局科技发展指导性计划基金(2005指导0584)

摘　　要：目的:观察转染了质粒PCI-NEO-SNCG后的前列腺癌激素非依赖性细胞系PC-3细胞对抗肿瘤药物顺铂(DDP)、5-氟尿嘧啶(5-FU)、阿霉素(ADM)、长春新碱(VCR)、紫杉醇(TAX)的敏感性,探讨SNCG的表达对各种抗肿瘤药物作用效果的影响。方法:转染质粒PCI-NEO和PCI-NEO-SNCG至PC-3细胞,采用RT-PCR法检测PC-3细胞中SNCG的表达;MTT法检测各抗肿瘤药物对转染后PC-3细胞的抑制作用;流式细胞术分析转染细胞经TAX作用后的细胞周期及凋亡。结果:5种抗肿瘤药物对转染了空载体PCI-NEO质粒及PCI-NEO-SNCG质粒细胞的生长抑制作用均存在时间依赖性;转染PCI-NEO的PC-3细胞组与转染PCI-NEO-SNCG的PC-3细胞组中各种抗肿瘤药物的抑制效果比较显示,DDP、5-FU、ADM、VCR的抑制效果两组间没有显著性差异(P>0.05),而TAX对转染PCI-NEO-SNCG的细胞的抑制率较转染PCI-NEO的细胞显著降低(P<0.01);经TAX处理48 h后,在转染PCI-NEO质粒的细胞中,停留在G2-M期的细胞比例显著高于转染PCI-NEO-SNCG质粒的细胞(P<0.01),而停留在G0-G1期及S期的细胞比例,在转染PCI-NEO质粒的细胞中显著低于转染PCI-NEO-SNCG质粒的细胞(P<0.01);在转染PCI-NEO质粒的细胞中Caspase-3的表达显著高于转染PCI-NEO-SNCG质粒的细胞(P<0.01)。结论:TAX对转染了SNCG基因的PC-3细胞中的生长抑制作用明显降低,提示SNCG的表达可抑制TAX的作用效果,这一发现可为前列腺癌的个体化治疗提供理论依据和指导。Objective： To observe the sensitivity of the PC-3 cell lines transfected with the PCI-NEO-SNCG plasmid to Cisplatin （ DDP）, 5-Fluorouraeil （5-FU）, Adriamyein （ADM）, Vinefistine （VCR） and Paclitaxel （ TAX）, and to explore the influence of the SNCG expression on the effectiveness of anti-tumor drugs. Methods： The plasmids PCI-NEO and PCI-NEO-SNCG were transfected into the hormone-independent prostate cancer cell lines PC-3. RT-PCR was adopted to examine the expression of SNCG in the PC-3 cell lines. The MTr method was employed to detect the suppressive effects of different anti-tumor drugs （ DDP, ADM, 5-FU, VCR and TAX） on the cell lines transfected with PCI-NEO and PCI-NEO-SNCG. Flow cytometry was used to analyze the cell cycles and apoptosis of the transfected cells treated with TAX. Results ： The 5 anti-tumor drugs suppressed the growth of the cell lines transfected with the plasmids PCI-NEO and PCI-NEO-SNCG in a time-dependant manner. The comparison between the growth-suppressing effects of different anti-tumor drugs on the PC-3 cell lines showed no significant differences between the group transfected with PCI-NEO and that with PCI-NEO-SNCG in DDP, 5-FU, ADM and VCR （P 〉 0.05）, while the rate of suppression of TAX on the latter cell lines was significantly lower than that on the former （P 〈 0.01 ）. Compared with the PCI-NEO-SNCG plasmid transfected cell lines, after treated with TAX for 48 hours, those transfected with the PCI-NEO plasmid exhibited a significantly larger proportion of cells remaining in the G2-M stage （P 〈 0.01 ）, a smaller proportion in the G0-G1 and S stages （P 〈 0.01 ） and a significantly higher expression of Caspase- 3 （P 〈 0. 01 ）. Conclusion： The significant reduction of the growth-suppressing effect of TAX in the SNCG-transfected PC-3 cell lines suggests that the expression of SNCG may restrain the effect of TAX. These findings have provided evidence and guide to the individual chemotherapy of prostate cancer.

关 键 词：SNCG基因 前列腺癌 PC-3细胞 转染 紫杉醇 

分 类 号：R737.25[医药卫生—肿瘤]