ADAM28反义核酸对人牙囊细胞生物学特性的影响  

Effects of ADAM 28 AS-ODN on Biological Characteristics of HDFCs.

作　　者：赵征[1] 徐军明[2] 赵威丽[2] 黄征难[1] 

机构地区：[1]解放军第401医院口腔科,山东青岛266071 [2]济南军区青岛第二疗养院

出　　处：《口腔医学研究》2008年第6期605-610,共6页Journal of Oral Science Research

基　　金：国家自然科学基金资助(编号:30572046)

摘　　要：目的:研究ADAM28反义核酸(AS-ODN)对人牙囊细胞(HDFC)增殖、分化、凋亡等生物学特性的影响。方法:采用细胞培养、基因转染、四唑盐(MTT)比色法、酶动力学方法和流式细胞术(FCM)检测ADAM28反义核酸转染HDFC后对细胞生物学特性的影响。结果:ADAM28 AS-ODN组HDFC的增殖活性、增殖指数、碱性磷酸酶(ALP)分泌活性显著降低,凋亡细胞百分比明显上升。结论:ADAM28反义核酸可显著抑制HDFC的增殖和ALP的活性,显著诱导HDFC的凋亡并影响细胞周期的变化。Objective： To study the effects of ADAM28 antisense oligodeoxynucleotide （AS -ODN） on biological char- acteristics of human dental follicle cells （HDFC） such as proliferation, differentiation and apoptosis. Methods： Cell culture, gene transfection, MTT chromatometry, enzyme dynamics, and flow cytometry （FCM） techniques were used to detect the effects of ADAM28 AS - ODN on biological characteristics of HDFC after transfected into HDFC. Results ： In ADAM28 AS -ODN group, proliferation activity, proliferation index and alkaline phosphatase（ALP） secretion activity of HDFC decreased significantly, and percentage of cell apoptosis were up - regulated obviously. Conclusion： ADAM28 AS - ODN could inhibit the proliferation and ALP activity of HDFC significantly, induce apoptosis of HDFC and influence the changes of cell cycle significantly.

关 键 词：ADAM28 反义核酸 人牙囊细胞 增殖 分化 凋亡 

分 类 号：R78[医药卫生—口腔医学]