β2肾上腺素受体在口腔鳞癌中的表达研究  

Expression of β2-Adrenergic Receptor in Oral Squamous Cell Carcinoma.

作　　者：尚政军[1] 刘克[1] 梁德凤[1] 

机构地区：[1]武汉大学口腔医学院口腔生物医学工程教育部重点实验室,湖北武汉430079

出　　处：《口腔医学研究》2008年第6期632-635,共4页Journal of Oral Science Research

基　　金：教育部"新世纪优秀人才支持计划"(编号:NCET-05-062)

摘　　要：目的:探讨β2-AR在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达及其在口腔鳞癌淋巴结转移中的作用。方法:采用免疫组化染色、RT-PCR和Western blot,检测β2-AR在65例口腔鳞癌组织、10例正常口腔黏膜组织、Tca8113细胞系中的表达,比较β2-AR表达与口腔鳞癌临床病理参数之间的关系。结果:口腔鳞癌组织中β2-AR的表达与正常口腔黏膜组织中β2-AR的表达之间存在显著差异(P=0.004)。在口腔鳞癌中,有淋巴结转移者的β2-AR阳性率为85.3%,无淋巴结转移者的阳性率为48.3%,两者之间的差异具有显著性(P=0.001)。RT-PCR和Western检测表明,口腔鳞癌组织和Tca8113细胞系中均有β2-AR表达,而正常口腔黏膜组织中无β2-AR表达。结论:β2-AR在口腔鳞癌中的表达显著升高,可能在口腔鳞癌发生和淋巴结转移中发挥一定的作用。Objective： To investigate the expression and possible roles of β2-adrenergic receptor（ β2-AR） in tumor development and metastasis in oral squamous cell carcinoma（OSCC）. Methods： By using immunohistochemistry, Western blot and RT - PCR, expression of β2- AR was examined in 65 cases of OSCC, 10 cases of normal oral mucosa, and Tca8113 cell line. The differences were statistically evaluated between β2-AR expression and various clinicopathological factors. Results ： It revealed a significant different positive 132 - AR expression between OSCC and normal controls （ P = 0. 004）. In OSCC, positive β2-AR expression was 85.3% in metastasis group vs. 48.3% in nonmetastasis group（P = 0.001 ）. RT- PCR and Western blot also confirmed positive 132 -AR expression in OSCC and Tca8113 cell line, and negative 132 - AR expression in normal oral mucosa. Conclusion： Increased expression of β2- AR may play an important role in the formation and metastasis of oral squamous cell carcinoma.

关 键 词：口腔鳞状细胞癌 肿瘤转移神经递质 Β2肾上腺素受体 

分 类 号：R739.86[医药卫生—肿瘤]