非小细胞肺癌凋亡相关基因与神经内分泌分化表达的研究  

Expression of Apoptosis Related Genes and Neuroendocrine Differentiation in Non-Small Cell Lung Carcinoma

作　　者：刘岩[1] 徐美林[1] 王菁[1] 杨霞[1] 

机构地区：[1]天津市胸科医院病理科,300051

出　　处：《天津医药》2008年第12期919-922,I0010,共5页Tianjin Medical Journal

摘　　要：目的:探讨非小细胞肺癌神经内分泌分化特征与细胞凋亡及凋亡相关基因Apaf-1和Caspase-3表达之间的关系。方法:对100例非小细胞肺癌手术标本应用TUNEL法检测细胞凋亡,免疫组织化学SP法检测癌组织中神经内分泌标志物神经特异性烯醇化酶、嗜铬素A、突触素及凋亡相关基因Apaf-1、Caspase-3蛋白的表达。结果:30例非小细胞肺癌具有神经内分泌分化特征,其平均凋亡指数及Apaf-1、Caspase-3蛋白表达均显著高于不伴神经内分泌分化者(P<0.05或P<0.01);Apaf-1和Caspase-3表达之间呈正相关(r=0.2877,P<0.01),两者与细胞凋亡之间亦呈正相关关系(r分别为0.3859和0.2316,均P<0.05)。腺癌和腺鳞癌组织的Apaf-1蛋白表达阳性率较高(P<0.01),Caspase-3蛋白表达阳性率在老年患者、中高分化癌和伴淋巴结转移者较高(P<0.05或P<0.01)。结论:伴神经内分泌分化的非小细胞肺癌具有较高凋亡活性,是其对放、化疗敏感的生物学基础,Apaf-1和Caspase-3高表达发挥了重要作用。Objective： To observe the relationship between the apoptosis and the neuroendrocrine differentiation in nonsmall cell lung carcinoma （NSCLC）, as well as the expression of the apoptosis related genes Apaf-1 and caspase-3. Methods： One hundred cases of non-small cell lung carcinoma were examined using immunohistochemical （IHC） staining for neuroendocrine markers （chromogranin A, synaptophysin and neuron specific enolase）, Apaf-1 and caspase-3 protein. Apoptosis of non-small cell lung carcinoma cells was detected by TUNEL technique. Results： Thirty cases were considered as NSCLC with neuroendocrine differentiation by the extensive and intensive staining of neuroendocrine markers, with higher apoptosis index and expression of Apaf-1 and caspase-3. Positive correlation was observed between the expression of Apaf-1 and caspase-3, as well as between either of them and apoptosis index. Apaf-1 was stained more strongly in adenoearcinoma and adenosquamous carcinoma. In contrast, caspase-3 expressed more intensively in the elders, mediate to well differentiation tumors and those with lymph node metastasis. Conclusion： Non-small cell lung carcinoma with neuroendoerine differentiation showed up-regulated apoptotic potential, which may contribute to its biological behavior such as the response to chemotherapy or radiotherapy. NSCLC expressed increased levels of Apaf-1 in mitochondria mediated apoptosome pathway and down- stream effecter caspase-3.

关 键 词：癌 非小细胞肺 神经分泌系统 细胞凋亡 基因 免疫组织化学 半胱氨酸天冬氨酸蛋白酶 

分 类 号：R734.2[医药卫生—肿瘤] R735.2[医药卫生—临床医学]