丹参酮对两种学习记忆功能障碍模型大鼠治疗作用的实验研究  

Curative Effectiveness of Tanshinone in the Treatment of Model Rats with Two Kinds of Learning and Memory Impairment

作　　者：李林[1] 夏保芦[1] 茹立强[2] 

机构地区：[1]江汉大学医学院组织胚胎学教研室,武汉430056 [2]华中科技大学同济医学院基础医学院神经生物学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2008年第6期819-822,827,共5页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省卫生厅科研基金资助项目(No.2005-JX2C35)

摘　　要：目的研究丹参酮对拟人类阿尔茨海默病(AD)和血管性痴呆(VD)2种模型大鼠学习、记忆功能障碍的改善作用。方法①实验动物随机分成5组:痴呆(AD/VD)模型组、丹参酮治疗组、假造模对照组和正常对照组。VD模型组:应用改良的大脑中动脉线栓塞(MCAO)法建模;AD模型组:应用D-半乳糖腹腔注射和海马内注射β-淀粉样肽蛋白片段1-40(Aβ1-40)复合造模方法建模。丹参酮治疗组:VD大鼠造模完成后或AD大鼠造模24h后给予丹参酮[50mg/(kg·d)],溶于5ml玉米油中灌胃14d。假造模对照组:与VD大鼠同法,但不做插线、结扎;与AD大鼠同法注射等量溶媒。正常对照组:大鼠不做任何处理。②采用水迷宫行为学实验,以单位时间逃避潜伏期及其频率为指标,测定大鼠空间学习、记忆功能。结果①AD/VD大鼠逃避潜伏期明显延长,逃生错误频率高,与两个对照组比较差异均有显著性意义。②丹参酮治疗组大鼠较痴呆模型组逃避潜伏期明显缩短,逃生错误频率减低,差异有统计学意义。结论①2种痴呆大鼠模型具有较好仿真人类AD/VD的特点。②丹参酮对AD/VD具有一定的治疗作用。Objective To study the improvement of learning and memory impairment in rat models of Alzheimer＇s-like disease （AD） and vascular-like dementia （VD） with tanshinone. Methods （1）The experimental animals were randomly divided into 5 groups： dementia （AD/VD） model group, tanshinone-treated group, false model group, and normal control group. In VD model group, modified middle cerebral artery occlusion （MCAO） method was used to create the modek Rat AD model was established by intraperitoneal injection of D-galactose in combination with microinjection of amyloid β-peptide 1-40 （Aβ1-40 ） into hippocampus. In the tanshinone-treated group, immediately after establishment of rat VD models or 24 h after creation of rat AD models, tanshinone [50 mg/（kg·day）] dissolved in 5 ml maize oil was given for 14 days by gastric perfusion. In false model group, the rats were treated with the same method as VD rats but without insertion of nynon thread and ligation, and with the same method as AD rats but with injection of the same volume of solvent. In normal control group, the rats were not treated. （2）By using a water-maze test, escape latency and frequency of escape failure were used as the indicators to assess learning and memory function of rats. Results （1）Escape latency of AD/VD rats was obviously prolonged, and frequency of escape failure was increased significantly as compared with two control groups; （2）Escape latency was obviously shortened and frequency of escape failure was decreased in tanshinone-treated group as compared with AD/VD model groups. Conclusion （1） Two kinds of dementia rat models have preferably characters of AD-like and VD-like; （2）Tanshinone treats AD/VD to some extent.

关 键 词：丹参酮 阿尔茨海默病 血管性痴呆 动物模型 水迷宫实验 学习记忆 

分 类 号：R749.16[医药卫生—神经病学与精神病学]