神经细胞核蛋白质与淀粉样前体蛋白基因启动子IL-Ⅰ反应元件相互作用研究  

The Interaction between Nuclear Protein and IL Ⅰ Response Elements of APP Protein in Neuronal Cell

作　　者：王少文[1] 马康涛[1] 宋宇蓓 张风云 张乃蘅 

机构地区：[1]北京医科学大学生物化学与分子生物学系

出　　处：《中国生物化学与分子生物学报》1998年第1期52-56,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家教委博士点基金

摘　　要：研究发现与ＩＬ－１诱导反应相关的ＡＰＰ启动子－４８８／－３０３片段，可与ＳＨ－ＳＹ５Ｙ细胞核抽提物中的核蛋白发生结合作用，并且在ＩＬ－Ⅰ刺激前后有量的变化，ＩＬ－Ⅰ刺激后其结合作用明显加强．冷竞争实验表明，此结合作用具有特异性．同时发现ＡＰＰ启动子－４８８／－３０３片段中的ＡＰ－Ⅰ作用位点及ＳＨ－ＳＹ５Ｙ细胞核抽提物中的ＡＰ－Ⅰ作用因子可能与此结合反应无关，而一个９０ｋＤ蛋白质与此结合反应有关．Previous studies have demonstrated that the expression of APP gene was increased in the SH SY5Y cell by induced of IL Ⅰ.Gel shift data indicate that the fragment of APP promoter -488/-303,which has been implicated as potential response element in the induction of APP transcription by IL Ⅰ,may interact with nuclear protein extracted from SH SY5Y cell.IL Ⅰ induction cause some modulation in amount of bound protein.Gel shift self competition assay showed that the binding is specific and expression of APP gene is not regulated efficiently by AP Ⅰ factor binding AP Ⅰ site.Instead,Southwestern blot result showed that a 90 kD protein may play a more important role.The data suggest that a 90 kD protein involve the regulation of APP gene expression in the SH SY5Y cell by induced of IL Ⅰ .

关 键 词：老年性疾呆 白细胞介素I 神经细胞核 

分 类 号：R749.16[医药卫生—神经病学与精神病学]