促黄体生成激素释放激素拮抗类似物的分离纯化研究  

Study on Separation and Purification of LHRH Antagonists by HPLC

作　　者：刘苗苗[1] 

机构地区：[1]天津市计划生育研究所药物室,天津300191

出　　处：《中国药业》2009年第1期7-8,共2页China Pharmaceuticals

摘　　要：目的采用高效液相色谱(HPLC)法分离纯化促黄体生成激素释放激素(LHRH)拮抗类似物。方法制备型色谱柱为Dynamax-300A型C18柱(250mm×4.14mm,5μm),分析型色谱柱为Nucleosil型C18柱(250mm×5mm,5μm);流动相A为含0.1%三氟醋酸和3%乙腈的水溶液(pH=2),流动相B为含70%乙腈的水溶液;流速为40mL/min(用于分离)和1.2mL/min(用于分析);检测波长为220nm。结果LHRH拮抗类似物纯品的产率可达22%～52%,纯度高达97%～99%,分离度大于1.5。结论HPLC法分离纯化的LHRH拮抗类似物纯品产率大,纯度高,且该法操作简便、快捷。Objective To inquire into the separation and purification of luneinizing hormone releasing hormone （LHRH） antagonists by HPLC. Methods HPLC was used. The preparative type column （Dynamax-300A C18, 250 mm ×4. 14 mm, 5 μm） and analytical type column （Nucleosil C18, 250mm×5 mm, 5 μm）;the aqueous solution of 1% trifluoroacetic acid and 3% acetonile as the mobile phase A（pH=2）, and the aqueous solution of 70% acetonilc as the mobile phase B; the flow rate was 40.0 mL/min （for separation） and 1.2 mL/min （for analysis）, the detecting wavelength was 220 nm. Results The product yield of purification was 22%-5267o and the purity was 97%- 99%, the resolution was large than 1.5. Conclusion The method is simple and fast. Therefore LHRH antagonists can be well purified by the way of HPLC.

关 键 词：高效液相色谱法 分离纯化 促黄体生成激素释放激素拮抗类似物 

分 类 号：R914[医药卫生—药物化学] R978.16[医药卫生—药学]