鸭肝金属硫蛋白的分离纯化及鉴定  

The Separation,Purification and Identification of Duck Liver's Metallothionein

作　　者：李福荣[1] 

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000

出　　处：《信阳师范学院学报（自然科学版）》2009年第1期118-120,共3页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：河南省科技攻关项目(0524240016)

摘　　要：以信阳华英鸭为材料,用ZnSO4诱导其肝脏合成金属硫蛋白(MT),将鸭肝材料经Sephadex G-50柱层析和DEAE-Sepharose CL-6B阴离子交换柱层析2次分离纯化,得到Zn-MT,该MT由61个氨基酸组成,其中半胱氨酸含量约为28%,分子量6.5 KD.鸭肝Zn-MT产率为0.57 mg/kg鲜肝重,在220 nm有吸收峰.The material of this experiment is Huaying duck from Xinyang area. The synthesis of metallothionein can be induced by ZnSO4 in the duck liver. The Zn-MT was isolated and purified by Sephadex G-50 column chromatography and DEAE-Sepharose CL-6B negative ion exchanging column. The protein is composed of 61 amino acids, including 28% of cysteine. The molecular weight of the protein is 6.5 KD, and the absorbing peak is shown at 220 nm. The yield rate of Zn-MT is 0. 57mg/kg of the fresh liver.

关 键 词：金属硫蛋白 鸭肝 锌 

分 类 号：Q503[生物学—生物化学]