藏獒犬α-干扰素全基因的克隆与序列分析  

Cloning and sequence analysis of interferon-α gene of Zangao canine

作　　者：陈红英[1,2] 董海聚[1] 崔保安[1,2] 贾艳艳[1,2] 宋亚朋[1,2] 王淑娟[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]河南省动物性食品安全重点实验室,郑州450002

出　　处：《安徽农业大学学报》2009年第1期124-127,共4页Journal of Anhui Agricultural University

基　　金：国家"十一五"科技支撑计划专项(2006BAD06A08)资助

摘　　要：根据GenBank发表的犬α-干扰素（IFN-α）基因核酸序列（AB102731），设计并合成1对引物，利用PCR技术从藏獒犬肝脏基因组DNA中扩增犬α-干扰素全基因，并进行克隆和测序。结果表明，获得了犬IFN-α全基因序列，其大小为564bp，包含1个完整阅读框，编码187个氨基酸残基。序列比较分析发现，克隆的藏獒犬IFN-α基因序列与GenBank中5条犬IFN-α基因序列核苷酸同源性在96．8％～99．3％之间，氨基酸同源性在94．7％-98．9％之间，并无插入和缺失变异，但是存在氨基酸点变异。藏獒犬与人和其他8种动物的IFN-α基因序列分析和系统进化分析表明，IFN-α基因存在种属的差异性，亲缘关系越近，同源性越高。犬IFN-α基因的成功克隆为进一步研究藏獒犬IFN-α基因表达、生物学活性和应用奠定基础。One pair of primers was desi （IFN-α） nucleic acid sequence （AB102731 ed and synthesized according to the canine interferon alpha gene published in the GenBank. Canine IFN-α gene was amplified by PCR from genome DNA extracted directionally from liver of Zangao canine, then cloned and sequenced. The result showed the full length sequence of Zangao canine IFN-α gene was consisted of 564 bp, which includes one openreading frame, encoding 187 amino acid residues, three potential N-glycosalation sites and ten cysteines in deduced amino acid sequence. The results of comparative sequence analysis and phylogenetic tree analysis showed there was difference of genus in IFN-α gene, the nearer relationship, the higher homology. This study set a basis the way for future study of biological function and application of canine IFN-α.

关 键 词：犬 α-干扰素基因 扩增 基因克隆 序列分析 

分 类 号：Q789[生物学—分子生物学]