土壤中抗真菌放线菌LH33发酵提取物活性研究  

Study on the inhibitive activity of the fermented extracts of soil of antifungal actinomycetes LH33

作　　者：刘欢[1] 安德荣[1] 慕小倩 张勤福 杨文军 

机构地区：[1]西北农林科技大学植物保护学院陕西省农业分子生物学重点实验室,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100 [3]陕西海浪生物研究所,陕西西安710000

出　　处：《西北农林科技大学学报（自然科学版）》2009年第1期145-150,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"863"高技术研究发展计划项目(2007AA021503);教育部"长江学者和创新团队发展计划"项目(200558)

摘　　要：【目的】对抗真菌放线菌LH33菌株发酵提取物的活性进行初步测定,为后续的生防制剂研发提供科学依据。【方法】制备从渭北旱塬轮作土壤中筛选出的放线菌菌株LH33的发酵提取物,检测其对番茄灰霉病菌孢子萌发的抑制作用及对番茄灰霉病的防治和番茄的促生作用,并采用生长速率法测定其抑菌谱。【结果】在离体条件下,LH33发酵提取物对番茄灰霉病菌孢子的萌发具有强烈的抑制作用。在温室条件下,LH33发酵提取物对蕃茄灰霉病具有很好的预防和治疗效果,100 mg/L的LH33发酵提取物对番茄灰霉病菌预防和治疗效果最高,分别为94.2%和80.4%。LH33提取物对大多数供试病原真菌都有很好的抑制效果,其中1 mg/L药液对部分病原真菌抑制率在45%以上。菌株LH33发酵提取物能促进番茄的生长,番茄植株鲜质量的增幅可达到95.1%。【结论】菌株LH33可防治水稻、番茄、小麦、棉花等多种植物真菌病,并对植物的生长有促进作用。[Objective] The study identified the enzymatic activity of fermented extracts of antifungal actinomycetes LH33 to provide a basis for the development of biological agents in the future. [Method] In this experiment,actinomycetes LH33 were identified and classified from rotation soils, which was conducted in the northern of Weihe River. Fermented extracts were prepared and its antifungal activity against tomato gray mould pathogen spores, and the antimicrobial spectrum as well as its growth-promoting effect were tested. [Result] Spore germination of tomato gray mould pathogen was strongly inhibited by LH33 fermented extracts in vitro. In greenhouse trails, the LH33 fermented extracts had good preventing and control efficiencies to botrytis cinerea,the rate of which was 94.2% and 80.45 respectively in 100 mg/L concentration. In addition,the active product of LH33 had a wide range of inhibition to pathogenic fungi tested here,the inhibition rate of fermented extracts （1mg/L）was up to 45% in some pathogens. Strain LH33 showed promoting effect on the tomato growth, the fresh weight of tomato increased 95.1% compared with CK. [Conclusion] Thus this antagonistic strain LH33 can control fungous disease of tomato, wheat, cotton and other plants, at the same time promotes the growth of plants.

关 键 词：拮抗放线菌LH33 发酵提取物 抑菌活性 真菌 

分 类 号：S432.43[农业科学—植物病理学]