人结直肠癌裸小鼠原位移植肝转移模型的建立及其生物学特性的研究  

Liver metastasis models of human colo rectal carcinoma established in nude mice by orthotopic transplantation and their biologic characteristics.

作　　者：刘秋珍[1,2] 脱朝伟[1,2] 吴秉铨[1,2] 张艳华[1,2] 

机构地区：[1]中国人民解放军第二○二医院肝胆普外科 [2]北京医科大学病理学系

出　　处：《中华肿瘤杂志》1998年第2期98-100,共3页Chinese Journal of Oncology

基　　金：军队科学基金

摘　　要：目的建立人结直肠癌裸鼠原位移植肝转移模型，为探讨人结直肠癌肝转移机制和抗转移治疗提供实验工具。方法采用组织学完整的人结直肠癌手术标本植入裸鼠结直肠（粘膜）壁内，观察原位移植的成瘤、移植瘤的侵袭和转移及其形态学特征（光镜、电镜、免疫组织化学）。结果从３４例人结直肠癌标本中筛选出一株人结肠癌裸鼠肝转移模型ＨＣＡ－ＨＭＮ－１，已传至１８代；一株人直肠癌裸鼠肝转移模型ＨＲＡ－ＨＭＮ－２，已传至２１代。共移植裸鼠２２８只，其移植生长率，肝转移率和液氮冻存复苏成活率均为１００％，表现为淋巴道、血道及种植转移。并具有分泌ＣＥＡ的功能。组织病理学和电镜观察，流式细胞仪ＤＮＡ含量测定及染色体核型的分析，结果证明与原始肿瘤完全一致。结论本研究所建立的两株人结直肠癌裸鼠肝转移模型，转移模式与临床患者相似。Objectives To establish a liver metastasis models of human colorectal carcinomas in nude mice. Methods Orthotopic transplantation of histologically intact colo rectal cancer tissues from patients into colo rectal mucosa of nude mice. Tumorgenicity, invasion, metastasis and morphological characteristics of the transplanted tumors were studied by light microscopy, electron microscopy and immunuhistochemistry. Results Liver metastasis models of human colon carcinoma (HCA HMN 1) and human rectal carcinoma (HRA HMN 2) were established after screening from 34 colorectal carcinomas. They had been passaged in vivo for 18 and 21 gererations respectively. There were lymphatic, hematogenous, and implanting metastases. CEA secretion was maintained after transplantation. The primary and liver metastatic tumors were similar to the original human carcinoma in histopathological and ultrastructural features, DNA content and chromosomal karyotype. Conclusion The liver metastasis models provide useful tools for the study of mechanism of metastasis and its treatment of human colo rectal cancer.

关 键 词：结肠肿瘤 直肠肿瘤 肝肿瘤 肿瘤移植 动物模型 

分 类 号：R735.3[医药卫生—肿瘤] R73－35[医药卫生—临床医学]