p63在口腔扁平苔藓中的表达  

Expression of p63 in oral lichen planus

作　　者：宋洁文[1] 程慧琳[2] 姚科[3] 蔡华雄[3] 

机构地区：[1]广州市第八人民医院口腔科,510060 [2]中山大学附属第二医院口腔科 [3]中山大学光华口腔医学院.附属口腔医院.口腔医学研究所

出　　处：《中华口腔医学研究杂志（电子版）》2008年第6期14-17,共4页Chinese Journal of Stomatological Research(Electronic Edition)

摘　　要：目的探讨p53家族新成员p63在口腔扁平苔藓(OLP)中的表达,其与OLP发生、发展的关系以及是否可作为一种标志物预测OLP的转归。方法采用免疫组织化学方法检测30例OLP中p63蛋白的表达,同时采用RT-PCR方法分析p63编码的两大类转录产物TAp63和ΔNp63的mRNA水平,并且与口腔鳞状细胞癌(OSCC)及正常口腔黏膜(NOM)进行比较。结果p63蛋白在NOM、OLP和OSCC组中均有表达,与NOM比较,其累积光密度(IOD)值在OLP中下调,在OSCC中明显上调。在糜烂萎缩型OLP中,p63蛋白表达高于非糜烂型(P<0.001)。RT-PCR检测结果显示,TAp63的mRNA水平在OLP中表现为中等,略低于NOM,明显高于OSCC;ΔNp63的mRNA水平在NOM和OLP中极低,其转录扩增带大多数检测不到,但在OSCC组都能检测到,而且呈高水平表达。TAp63的mRNA水平在糜烂萎缩型OLP中低于非糜烂型(P=0.018),ΔNp63则高于非糜烂型(P=0.049)。结论p63与OLP的发病机制有关,其中TAp63和ΔNp63分别起不同的作用。p63表达的高低可能可以作为预测OLP是否有高风险癌变的检测指标。Objective To investigate the expression of p63 in oral lichen planus （OLP） and to evaluate the role of p63 in the development of OLP and prediction of cancer change as a molecular marker. Methods Tissue specimens of 30 OLP were examined by immunohistochemistry and reverse transcriptase-polymerase chain reaction （RT-PCR） for the expression of p63. Results were compared with normal oral mucosa （NOM） and oral squamous cell carcinoma （OSCC）. Results p63 expression was detect in all specimens. Compared with NOM, the integrated option density （IOD） value was down regulated in OLP, while up regulated in OSCC. Atrophic-erosive OLP showed higher p63 expression than reticular OLP. TAp63 mRNA was moderately expressed in OLP, being lower than in NOM and significantly higher than in OSCC. mRNA transcripts for ANp63 almost could not be detected in NOM and OLP tissues. But in OSCC, ANp63 mRNA was recognized in all tissues and expressed at a high level. Conclusions p63 might be associated with the pathogenesis of OLP. TAp63 and ANp63 have different function in OLP tissues, p63 might be an early marker to predict the carcinomatous change risk of OLP.

关 键 词：口腔扁平苔藓 口腔鳞状细胞癌 P63 

分 类 号：R781.5[医药卫生—口腔医学]