尼莫地平对铝损伤大鼠脑组织MAO-B表达的影响  

Effect of nimodipine on the MAO-B expression in rat brain injuried by aluminum

作　　者：钟梁[1] 张静[2,3] 杨俊卿 周岐新[2,3] 

机构地区：[1]重庆医科大学临床检验诊断学教育部重点实验室,重庆400016 [2]重庆医科大学药学院药理教研室 [3]重庆市生物化学与分子药理学重点实验室

出　　处：《中国老年学杂志》2009年第1期20-22,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(30672211;30300449);重庆市科委自然科学基金(048528)

摘　　要：目的观察铝负荷大鼠脑组织单胺氧化酶B(MAO-B)的活性与表达改变,并探讨尼莫地平对该动物模型的影响。方法采用三氯化铝(AlCl3)(400mg/kg)灌胃大鼠,1次/d,5d/w,持续3个月,建立铝负荷致大鼠慢性脑损伤的动物模型,尼莫地平(80mg/kg)在每次铝给予4h后灌胃,观察行为学、生化酶学、MAO-B mRNA及蛋白表达水平等指标的变化。结果铝负荷能明显导致大鼠被动回避性学习记忆能力和空间识别能力障碍,使其脑组织乙酰胆碱转移酶(ChAT)活性显著降低(P<0.01),而胆碱酯酶(AchE)活性显著升高(P<0.01),超氧化物歧化酶(SOD)活性显著降低(P<0.01),而丙二醛(MDA)含量显著升高(P<0.01),MAO-B活性与MAO-B mRNA及蛋白表达水平显著增加(P<0.01);NMDP能明显改善铝负荷大鼠的学习记忆能力障碍,明显阻遏铝负荷大鼠海马组织ChAT与SOD活性的降低,AchE活性与MDA含量的增高,MAO-B活性及其mRNA与蛋白表达的增加。结论铝负荷致大鼠脑损伤涉及脑组织的MAO-B活性与表达改变;NMDP可能降低铝负荷大鼠脑组织MAO-B表达和活性,并增加SOD活性,减少自由基而保护大鼠慢性脑损伤。Objective To study the change of MAO-B activity and expression in brain tissue of aluminum-overload rat and the effect of nimodipine on this animal model. Methods The chronic brain injury model of rats were established by intragastric administration of aluminum trichloride （400 mg/kg） once daily,5 d/w for 3 months,and nimodipine （80 mg/kg） was intragastrically administered 4 h following aluminum administration. The changes of behavior, biochemical enzyme and the expression of MAO-B mRNA and protein in rat brain tissue were observed. Results Aluminum-overload markedly induced impairments of avoidant learning and memory function and spatial distinguish function in rats, while ChAT activities significantly decreased （ P 〈 0. 01 ）, but AchE activities increased （ P 〈 0. 01 ）, SOD activities signifi- cant decreased （P 〈0. 01 ）, but MDA contents increased （P 〈0. 01 ） ,MAO-B activities and expression of MAO-B mRNA and protein signif- icantly increased in rat brain tissue （P 〈 0. 01 ）. Conclusions The change of MAO-B activity and expression in brain tissue is involved in brain injury induced by aluminum-overload rat. Nimodipine has an obvious protective effect against chronic brain injury. The mechanism of the protective effect may be related to the decreasing of MAO-B activities and expression,increasing of SOD activities in bran tissue of aluminum- overload rat, thus leads to reduce the free radical.

关 键 词：尼莫地平 铝负荷大鼠 单胺氧化酶B 

分 类 号：R741.02[医药卫生—神经病学与精神病学]