SPP-1、GDF-15和CXCL-1在人小肝癌中表达的初步研究  

Initial Study on the Expression of SPP-1,GDF-15 and CXCL-1 in Human Small Hepatocellular Carcinoma

作　　者：查斌山[1] 刘开坤[1] 刘悦芳[2] 周顺[1] 朱进[2] 冯振卿[2] 王轩[3] 张建平[1] 

机构地区：[1]南京医科大学第二附属医院普通外科,江苏南京210011 [2]南京医科大学卫生部抗体技术重点实验室,江苏南京210029 [3]中国人民解放军第81医院肿瘤外科,江苏南京210002

出　　处：《武汉大学学报（医学版）》2009年第1期38-42,共5页Medical Journal of Wuhan University

基　　金：卫生部科学研究基金资助项目(编号:wkj2004-2-012);南京医科大学科技发展基金重点项目(编号:08NMUZ018)

摘　　要：目的:针对前期应用基因芯片(Affymetrix Rat Genome 230.2.0)筛选出的大鼠早期肝癌中高表达的人同源分泌蛋白基因SPP-1、GDF-15和CXCL-1,定量验证其在人小肝癌中mRNA和血清蛋白的表达水平,并探讨其临床应用价值。方法:应用实时定量PCR和双抗体夹心法ELISA,检测15例小肝癌及正常肝脏组织(癌旁正常肝脏组织)的mRNA水平及对应小肝癌血清和正常人血清中的蛋白表达水平。结果:①SPP-1、GDF-15和CXCL-1基因在小肝癌组织中较对照组表达显著上调,其差异有统计学意义(P<0.05);②SPP-1血清蛋白检测值与正常对照组比较差异无统计学意义(P>0.05);GDF-15血清蛋白含量在小肝癌组中表达水平明显高于正常对照组,差异有统计学意义(P<0.05);小肝癌组中CXCL-1血清蛋白含量较正常对照组表达下调,其差异有统计学意义(P<0.05)。结论:①NF-κB信号通路基因(CXCL-1)、P53信号通路基因(GDF-15)和SPP-1基因在人小肝癌组织中高表达,可能与肝癌发生的早期过程相关;②分泌性蛋白GDF-15和CXCL-1显示出小肝癌筛选诊断的潜在价值。Objective： To quantitative validate the expression of SPP-1, GDF-15 and CXCL-1 in small hepatocellular carcinoma and investigate the clinical value. Methods： The mRNA expression （of SPP-1, GDF-15 and CXCL-1 was determined by real-time PCR in samples from 15 small HCCs, including tumor tissues and their matched normal liver tissues. The protein expression was surveyed by double antibody sandwich ELISA in serum samples, including 15 cases of corresponding tumor patients and random healthy adults. Results： （1） The mRNA expression of SPP-1, GDF-15 and CXCL-1 significantly increased of small HCC tissues compared to the paired normal tissues （P〈0.05）. （2） Double antibody sandwich ELISA analysis revealed that GDF-15 protein was expressed higher in the serum in small HCC patients than in the control group （P〈0.05） and the difference was not significant between SPP-1 and control group（P）0.05） ; Expression of CXCL 1 revealed the opposite expression pattern of in the serum of small HC Cpatients （P〈0.05）. Conclusion： NF-κB signal pathway gene （CXCL-1）, P53 signal pathway gene （GDF-15）, and SPP-1 may be associated with the hepatocarcinogenesis; CXCL-1 and GDF-15 should be potential tumor markers in detecting HCCs at early stage.

关 键 词：小肝癌 肿瘤标志物 实时荧光定量PCR 双抗体夹心ELISA 

分 类 号：R735.7[医药卫生—肿瘤]