CD_(34)、CD_(105)免疫组化染色评价肺转移瘤肿瘤新生血管生成  

Appraisal of tumor-associated neoangiogenesis in pulmonary metastases with anti-34/anti-105 immunohistochemical staining

作　　者：吴安乐[1] 黄求理[1] 柴小民[1] 宋侃侃[1] 施建国[1] 居洁勤[1] 余峰[1] 

机构地区：[1]宁波市第一医院,浙江宁波315010

出　　处：《现代实用医学》2008年第12期928-931,共4页Modern Practical Medicine

基　　金：宁波市科技局基金资助项目;编号2007C10067

摘　　要：目的通过CD34、CD105免疫组化染色评价肺转移瘤肿瘤新生血管生成。方法将18例肺单发转移瘤外科切除标本分别行CD34、CD105血管内皮染色。通过高倍视野下测定微血管密度和微血管形态观察。结果CD34和CD105免疫组化染色阳性微血管密度分别为5.27±1.47和1.19±0.53。肝癌肺转移瘤和结直肠腺癌肺转移瘤CD105阳性微血管密度分别为1.25±0.68和1.16±0.42,差异无统计学意义(=0.36,>0.05)。CD34染色容易显示瘤内和瘤周的成熟肿瘤血管和肿瘤组织包绕的正常血管,而CD105染色能够显示瘤内活性新生血管。高倍镜下肿瘤血管包括分支状、窦隙状、芽孢状、圆形以及镶嵌状等多种形态。结论不同病理来源肺转移瘤均存在肿瘤血管生成,CD105能够显示瘤内活性增生肿瘤血管,是转移瘤新生肿瘤血管特异性指标,能够揭示转移瘤内新生血管形成。Objective To appraise tumor-associated neoangiogenesis of pulmonary metastatic nodules with CD34, CD105（Endoglin） immunohistochemical staining. Methods Surgical specimens from 18 patients with pulmonary metastatic nodules were immunostained with anti-CD34 mAb and anti-CD105 mAb respectively. Microvessel density （MVD） of positively stained microvessels in CD34 and endoglin staining was calculated in densely vascular foci（Hotspots）,respectively.In the meantime, MVD ofendoglin staining in the two different pathologically- originated metastases was compared. Results MVD ofCD34 immunostaining and endoglin staining was 5.27±1.47 and 1.19±0.53,respectively. There was no significance in MVD of pulmonary metastases for endoglin staining between the two different nodules originated from HCC and colorectal adenocarcinoma （1.25±0.68 vs 1.16±0.42, t=0.36, P〉0.05）. Tumor vessels might be divided into several components such as normal blood vessels, matured tumor vessels, mosaic vessels, activated proliferating vessels, and so on. CD34 was apt to stain all kinds ofmicrovessels. Nevertheless, endoglin significantly demonstrated more proliferating neoplastic microvessels including activated tumor vessels and mosaic vessels. Conclusions Different pathologically-originated pulmonary metastases equally presents large amount of tumor vessels due to tumor-associated angiogenesis. Endoglin immunostaining can demonstrate more activated proliferating tumor vessels. Endoglin may be one of specific markers aiming at activated neoplastic vessels in pulmonary metastatic nodules and can reveal angiogenesis in secondary metastatic site.

关 键 词：CD105 免疫组化染色 肺肿瘤 肿瘤转移 肿瘤血管生成 

分 类 号：R733[医药卫生—肿瘤]