脑星形细胞瘤CyclinD1、P21^(WAF1/CIP1)和Survivin关系的探讨  

Expression of the CyclinD1、P21^(WAF1/CIP1) and Survivin Gene in Human Astrocytoma and Their Correlation

作　　者：王淳良[1] 梅金红[2] 徐姗[2] 万红萍[2] 陈任生[2] 王珊珊[2] 徐林林[2] 

机构地区：[1]南昌大学第一附属医院神经外科,南昌330006 [2]南昌大学第一附属医院病理科,南昌330006

出　　处：《江西医学院学报》2008年第6期47-51,共5页Acta Academiae Medicinae Jiangxi

基　　金：江西省教育厅科学技术研究项目(200677)

摘　　要：目的探讨CyclinD1、P21WAF1/CIP1和Survivin与脑星形细胞瘤的相关性。方法采用ElivisionTMplus免疫组化方法,检测10例正常脑组织、60例脑星形细胞瘤中CyclinD1、P21WAF1/CIP1和Survivin的表达情况。结果在正常脑组织和脑星形细胞瘤Ⅰ级、Ⅱ级、Ⅲ级、Ⅳ级中的阳性表达情况:(1)CyclinD1阳性表达率分别为:20.00%(2/10)、25.00%(4/16)、43.75%(7/16)、50.00%(7/14)、71.43%(10/14),其表达与病理分级呈显著正相关(r=0.357,P<0.01);(2)P21WAF1/CIP1的阳性表达率分别为:0(0/10)、81.25%(13/16)、62.50%(10/16)、42.86%(6/14)、27.27%(3/14),其表达与病理分级呈显著负相关(r=-0.548,P<0.001);(3)Survivin的阳性表达率分别为:0(0/10)、56.25%(9/16)、68.75%(11/16)、92.86%(13/14)、100.00%(14/14),其表达与病理分级呈显著正相关(r=0.539,P<0.005);(4)CyclinD1表达阳性的28例中,P21WAF1/CIP1阳性表达11例,阳性率39.29%(11/28),Cy-clinD1表达阴性的32例中,P21WAF1/CIP1阳性表达21例,阳性率65.63%(21/32),两者呈负相关(r=-0.362,P<0.02);(5)Survivin表达阳性的47例中CyclinD1阳性表达27例,阳性率57.45%(27/47),Survivin表达阴性的13例中CyclinD1阳性表达1例,阳性率7.69%(1/13),两者呈显著正相关(r=0.411,P<0.005);(6)P21WAF1/CIP1表达阳性的32例中survivin阳性表达20例,阳性率62.50%(20/32),P21WAF1/CIP1表达阴性的28例中Survivin阳性表达27例,阳性率96.43%(27/28),两者呈显著负相关(r=-0.411,P<0.005)。结论(1)CyclinD1过表达、P21WAF1/CIP1表达减弱或消失以及Survivin的高表达与脑星形细胞瘤的发生、发展有关;(2)联合检测三者在一定程度上反映脑星形细胞瘤的生物学行为。Objective To discuss the dependability of CyclinD1. P21^WAF1/CIP1 and Survivin in human astrocompass. Methods ElivisionTMplus immunohistochemical staining method was used to detect the expression of CyclinD1. P21^WAF1/CIP1 Survivin in 60 human astrocytoma. Results The expression status in normal brain tissue and astrocytoma of grade Ⅰ .grade Ⅱ .grade Ⅲ and grade Ⅳ,（1） The CyclinD1 positive ratios were 20. 00% （2/10）, 25. 00% （4/16）, 43. 75% （7/16）, 50. 00% （7/14）, 71. 43% （10/14）, there was significant positive correlation difference between four grades（r=0. 357,P〈0.01）. （2）The P21^WAF1/CIP1 positive ratios were 0（0/10）,81.25%（13/16）,62.50%（10/16） ,42.86%（6/14）,27.27%（3/14） ,there was significant negative correlation difference between four grades （r = - 0. 548, P〈 0. 001）. （3） The Survivin positive ratios were 0（0/10）,56.25%（9/16）,68.75%（11/16）,92.86%（13/14）,100.00%（14/14）, there was significant positive correlation difference between four grades（r=0. 539, P〈0. 005）. （4）In 28 example positive expression of CyclinD1, there were 11 examples showing positive expression of P21^WAF1/CIP1 , the positive ratio was 39.29%（11/28） ,and in 32 example negative expression of Cy- clinD1, there were 21 examples showing positive expression of P21^WAF1/CIP1 , the positive ratio was 65. 63%（21/32）, there was negative correlation difference between CyclinD1 and P21^WAF1/CIP1 （r=-0. 362,P〈0.02）. （5）In 47 example positive expression of Survivin ,there were 27 examples showing positive expression of CyclinD1, the positive ratio was 57.45 % （27/47）, and in 13 examples showing negative expression of Survivin, there are 1 example showint positive expression of CyclinD1, the positive ratio was 7.69% （1/13）, there was significant positive correlation difference between CyclinD1 and Survivin（r= 0. 411, P〈0. 005）. （6） In 32 examples showing positive expression of P21^WAF1/CIP1 , there were 20 examples

关 键 词：脑星形细胞瘤CyclinD1、P21^WAF1/CIP1 SURVIVIN 免疫组化 

分 类 号：R739.41[医药卫生—肿瘤]