膀胱癌组织中ING1mRNA的表达与意义  

Expression and Significance of Tumor Suppressor Gene-ING1mRNA in Bladder Carcinoma

作　　者：吴高亮[1] 黄光毅[2] 习小庆[2] 

机构地区：[1]江西省肿瘤医院泌尿外科,南昌330029 [2]南昌大学第二附属医院泌尿外科,南昌330006

出　　处：《江西医学院学报》2008年第6期52-55,共4页Acta Academiae Medicinae Jiangxi

基　　金：江西省卫生厅课题(2003)

摘　　要：目的探讨抑癌基因ING1mRNA在膀胱癌组织中的表达及与肿瘤发生发展的关系。方法采用RT-PCR方法检测38例膀胱癌手术患者的癌组织及其周围正常组织中抑癌基因ING1mRNA的表达水平。结果癌周组织中ING1mRNA均阳性表达;32例膀胱癌组织中ING1mRNA的表达较正常组织降低(32/38,84.2%),其中无表达2例;癌组织表达强度与正常组织相比差异显著(P<0.01),其低表达与肿瘤分级、分期及淋巴转移有关。结论ING1mRNA表达在膀胱癌发展过程中可能发挥重要的调节作用。ING1mRNA可能是膀胱癌患者潜在的恶性判断指标及诊断、预后的判断指标。Objective To explore the expression of ING1 in bladder carcinomas, and evaluate the relationship between the expression level and the progression of tumor. Methods The specimens from 38 patients including cancer tissues and normal tissue around tumors were detected for their expression levels of ING1 mRNA by RT-PCR. Results The positive expression of ING1 mRNA was found in all normal tissues around tumors. A significant decrease in ING1 mRNA was found in 32 out of 38 bladder carcinomas （32/38,84.2%）, and 2 specimens were found no expression. The expression level in tumors was different significantly from that in normal tissues （P〈0. 01）. It showed that repression of INGlmRNA expression might be correlated to the stage,grade and metastasis. Conclusion Down-regulation of ING1 may play an important role in the oncogenesis and development of bladder carcinomas. It may be used as an candidate indicator for malignity-judgement, diagnosis and prognosis of bladder carcinomas.

关 键 词：膀胱肿瘤 抑癌基因 INGI 逆转录聚合酶链反应 

分 类 号：R737.14[医药卫生—肿瘤]