细粒棘球蚴egG1Y162抗原基因的克隆及蛋白质序列分析  

Cloning and protein sequence analysis of the egG1Y162 gene from Echinococcus granulosus

作　　者：曹春宝[1,2] 马秀敏[1,2] 丁剑冰[1,2] 贾海英[1,2] 吾拉木·马木提[1,2] 张海涛[1,2] 朱明[2] 马海梅[1,2] 吕国栋[1] 温浩[1] 

机构地区：[1]新疆医科大学第一附属医院,新疆包虫病VIP实验室,新疆乌鲁木齐830011 [2]新疆医科大学基础医学院

出　　处：《中国病原生物学杂志》2008年第12期903-906,共4页Journal of Pathogen Biology

基　　金：国家自然科学基金资助项目(No.30560146;30860263);国家教育部春晖计划(No.Z2004-2-65004)

摘　　要：目的克隆细粒棘球蚴egG1Y162基因序列并进行蛋白序列对比分析。方法从细粒棘球蚴原头蚴提取mRNA,mRNA反转录为cDNA。设计特异引物,以cDNA为模板扩增egG1Y162基因,构建PUCm-T/egG1Y162重组质粒,经PCR、酶切及测序鉴定后进行序列分析。结果egG1Y162 cDNA为459bp,编码153个氨基酸;同源性比较表明,egG1Y162 cDNA与emY162同源性为95%,与eg95的同源性为30.61%。结论成功克隆egG1Y162抗原基因,egG1Y162蛋白质氨基酸序列与emY162有很高的相似性,但同其他抗原蛋白质序列存在明显差异,所以egG1Y162是一种新的抗原基因。Objective To clone the egG1Y162 gene and analyze the protein sequence. Methods mRNA was extracted from protoscolex of Echinococcus granulosus and used for RT-PCR. The primers of egG1Y162 were designed according to the sequence of emy162 antigen genes. Using cDNA as a template, egG1Y162 gene was amplified by PCR, and recombinant plasmid PUCm-T/egG1Y162 was constructed. Then recombinant plasmid was identified by PCR, enzyme digestion and sequencing. Results The length of the egG1Y162 cDNA was 459 bp, which coded 153 aa. Homology comparison showed that the egG1Y162 cDNA sequence shared 95% homology with emY162 gene, while shared 30.61% homology with eg95. Conclusion egG1Y162 antigen gene is a new gene and has been cloned successfully. Comparative analysis showes that amino acid sequences of egG1Y162 protein is similar to that of egY162 and significant differences existed in the amino acid sequences of egG1Y162 protein as compared with others antigen proteins.

关 键 词：细粒棘球蚴 egG1Y162 CDNA 序列分析 

分 类 号：R383.33[医药卫生—医学寄生虫学]