锌对前列腺癌PC-3M细胞侵袭能力的影响  

The effect of zinc on the invasion ability in human prostate carcinoma cell PC-3M

作　　者：汲坤[1] 张灵[1] 李馨[1] 王波[1] 彭博[1] 李晓萌[1] 李扬[1] 赵雪俭[1] 

机构地区：[1]吉林大学基础医学院病理生理教研室暨吉林大学前列腺疾病防治研究中心,吉林长春130021

出　　处：《中国实验诊断学》2009年第1期47-51,共5页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨锌对人前列腺癌PC-3M细胞侵袭能力的影响。方法用MTT比色法检测不同剂量锌对PC-3M细胞增殖的影响;Transwell小室检测锌对PC-3M细胞侵袭能力的影响;RT-PCR检测锌作用24 h后MMP-2、MMP-9 mR-NA的表达;酶谱分析检测锌作用后PC-3M细胞MMP-2、MMP-9的活性变化。结果MTT检测结果表明一定剂量的锌可抑制PC-3M细胞的生长;Transwell小室实验结果显示,锌作用下PC-3M细胞穿透Matrigel膜的细胞数减少;RT-PCR结果显示锌抑制了PC-3M细胞MMP-2和MMP-9的表达;酶谱分析表明锌作用后PC-3M细胞MMP-2、MMP-9的活性下降。结论一定剂量的锌可降低PC-3M细胞的侵袭能力,其机制可能与抑制MMP-2、MMP-9的合成和激活有关。Objective To investigate the effects of zinc on the mRNA expression of MMP-2 and MMP-9 of human prostate carcinoma cell PC-3M. Methods The effects of zinc with different concentractions on proliferation of PC-3M cell were detemained by MTT assay. Transwell chamber was used to investigate the effects of zinc on invasion abilities of PC-3M cell. The mRNA expression of MMP- 2 and MMP-9 were detected by RT-PCR synthesis after 24h in culture with zinc. The activation of MMP-2 and MMP-9 was detected by gelatin zynography. Results MTr assay showed that zinc with a certain concertration could inhibit proliferation of PC-3M cell. Under the effect of zinc,the number of PC-3M cell which moved through the matrigel membrane decrease.The result of RT-PCR and gelatin zynography respectively showed the synthesis and activation of MMP-2 and MMP-9 decreased after treated with zine.d. Conclusion Zinc with a certain concentraction can inhibit the proliferation and invasion abilities of PG-3M cell,and the mechanism is possibly relevant to the inhibitation of the synthesis and activation of MMP-2 and MMP-9.

关 键 词：锌 前列腺癌 基质金属蛋白酶2 9 PC-3M 

分 类 号：R737.25[医药卫生—肿瘤]