河流沉积物中反硝化细菌的分离及脱氮除磷研究  

Denitrifying Bacteria Isolated from River Sediment and Its Nitrogen and Phosphorus Removal Capacity from River Water

作　　者：王琳[1,2] 李季[2] 康文力[2] 陈云增[1] 郭廷忠[1] 

机构地区：[1]河南大学环境与健康工程研究中心,开封475001 [2]中国农业大学资源与环境学院,北京100094

出　　处：《环境科学》2009年第1期91-95,共5页Environmental Science

基　　金：“十一五”国家科技支撑计划项目(2006BAD17B05);北京市生态学重点学科项目(XK10019440);北京市都市农业学科群建设项目(XK100190553)

摘　　要：采用常规细菌分离方法,从河流沉积物中筛选出20株具有反硝化作用的细菌菌株,研究了其反硝化强度,对反硝化强度最大的菌株进行了鉴定,并进一步研究了其不同浓度下脱氮除磷的性能.结果表明,筛选的菌株均具有一定的脱氮能力,但不同菌株的脱氮能力不同,反硝化强度在50%以上的有10株,其中F10菌株的脱氮能力最强为63.2%,通过形态学、革兰氏染色结合16S rDNA序列同源性分析鉴定,其鉴定结果为粪产碱杆菌(Alcaligenes faecalis);不同浓度的F10净化生活污水,其中100mg/L的处理效果最好,在第10d时,总氮、总磷的去除率最大,分别为76.2%、93.8%.20 strains of bacteria were isolated from river sediment using enrichment culture medium for denitrification, and the denitrification intensity was determined. F10, one of bacteria strain, was identified having the highest denitrifying intensity, and further used to test its role in the removing of nitrogen and phosphorus from wastewater in laboratory. By checking the individual morphology, colony culture characteristics, DNA sequencing and 16S rDNA gene bank, F10 was identified as Alcaligenes faecalis, and its denitrifying intensity was 63.2% . The highest removal of TN （76.2%） and TP （93.8%） were observed in a 10 days period with an addition of F10 at a rate of 100 mg/L.

关 键 词：反硝化细菌 富营养化 河流沉积物 生活污水 生物修复 

分 类 号：X172[环境科学与工程—环境科学]