鉴定马铃薯染色体数目的方法研究  

Study on Identified Method of Chromosome Count of Potato

作　　者：王舰[1,2] 杨永智[1,2] 蒲秀琴[1,2] 

机构地区：[1]青海省农林科学院生物所,青海西宁810016 [2]青藏高原生物技术教育部重点实验室,青海西宁810016

出　　处：《黑龙江农业科学》2009年第1期1-2,共2页Heilongjiang Agricultural Sciences

摘　　要：用块茎根尖、试管苗根尖及组培移栽苗匍匐茎尖为材料鉴定马铃薯的倍性，结果表明：利用匍匐茎尖作为材料，用饱和的对二氯溶液处理10-12h后，用改良的卡洛氏固定液固定24h，用1M的HCl60℃解离10-15min，进行染色15-20min，效果很好，表明在不易取材或材料稀少的情况下，用匍匐茎尖制片是一个鉴定马铃薯染色体数的有效经济的方法。The potato-ploidy was identified from the creeping rootstock,foot tip and foot tip of tissue culture respectively. These issue rootstocks were treated by Colchicine,8-Hydroxyquinoline, saturated 1,4-dichlorobenzene and ice-water for 12h,fixed by improved Cacnoy for 24 h,later dispelled for 10-15 min, stained by Carbol fuehsin 15-20 rain, the results showed that the method that creeping rootstock was better material,it could be treated better by 1,4-diehlorobenzene When there had many germplasm resources need to identify but rare material was better.

关 键 词：马铃薯 种质资源 倍性鉴定 

分 类 号：S532[农业科学—作物学]