清肝化痰活血方对非酒精性脂肪性肝炎模型大鼠的干预作用  

Qinggan Huatan Huoxue Formula on the model of rats with nonalcoholic steatohepatitis

作　　者：裴强[1] 王晓素[2] 李英[2] 

机构地区：[1]新乡医学院第一附属医院中西医结合科,河南卫辉453100 [2]上海中医药大学附属岳阳中西医结合医院消化科,上海200437

出　　处：《新乡医学院学报》2009年第1期25-28,共4页Journal of Xinxiang Medical University

基　　金：上海市卫生局课题(编号:2004L003A)

摘　　要：目的观察清肝化痰活血方对非酒精性脂肪性肝炎(NASH)模型大鼠的影响,并探讨其作用机制。方法雄性SD大鼠36只分为对照组6只、模型组10只、清肝化痰活血方干预组(清肝组)10只、胆宁片干预组(胆宁组)10只。对照组普通饲料喂养;其余3组均采用高脂饮食复制NASH动物模型,清肝组、胆宁组分别给予清肝化痰活血方药液和胆宁片混悬液灌胃,对照组和模型组同时予生理盐水灌胃。造模12周末处死各组大鼠,检测体质量、肝指数、炎症活动度计分、血清丙氨酸氨基转氨酶(ALT)、门冬氨酸氨基转氨酶(AST)、γ-谷氨酰转移酶(γ-GT)、总胆固醇(TC)、甘油三酯(TG)、游离脂肪酸(FFA)含量和肝组织中TG、FFA、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性。结果第12周时模型组和胆宁组大鼠体质量、肝指数、炎症活动度计分、血清AST、ALT、γ-GT、TG、TC、FFA水平、肝组织TG、MDA、FFA含量均高于对照组和清肝组(P<0.05),SOD活性均低于对照组和清肝组(P<0.05);胆宁组体质量、肝指数、炎症活动度计分及血清ALT、TG、TC和FFA水平、肝组织TG、FFA均较模型组降低(P<0.05);与对照组比较,清肝组炎症活动度计分、血清TG增高(P<0.05),肝组织SOD活性降低(P<0.05)。结论清肝化痰活血方对NASH模型大鼠有良好的防治作用,其作用机制可能是通过调节脂质代谢,减轻脂类在肝脏的积聚,抗脂质过氧化,缓解氧化应激。Objective To observe the preventative and therapeutic effects of Qinggan Huatan Huoxue formula （QGHTHXF） on rat models with nonalcoholic steatohepatitis,and to investigate its possible mechanism. Methods 36 male SD rats were divided into four groups：6 rats were fed by control food as the control group. 10 rats in model group, 10 rats in QGHTHXF group and 10 rats in Danning group were fed with high-fat diets in order to establish the nonalcoholic steatohepatitis model. The rats in QGHTHXF group and Danning group were given QGHTHXF physic liquor and Danning suspension by intragastric administration respectively. Control group and model group were given physiological saline by intragastric administration. The rats in each group were killed at the end of the twelfth week ＇after established the model. Body mass, liver index, inflammation score and alanine aminotransferase （ALT） , aspartate transaminase （ AST ） , γ-glutamyhransferase （ γ-GT ）, total cholesterol （ TC ） , triglyceride （ TG ）, free fatty acid （ FFA ） in serum and TG, FFA, malondialdehyde （ MDA ）, superoxide dismutase（SOD） in hepatic tissues were detected. Results At the end of the twelfth week, the body mass, liver index, inflammation score and ALT, AST, γ-GT, TG, TC, FFA in serum and TG, FFA, MDA in hepatic tissues in model group and Danning group were higher,but the activity of SOD was lower than that in control group and QGHTHXF group（ P 〈 0. 05 ）. The body mass,liver index, inflammation score and ALT, TG, TC, FFA in serum and TG, FFA in hepatic tissues in Danning group were lower than those in model group（P 〈 0.05 ）. Compared with control group,the inflammation score and TG were higher, but the activity of SOD was lower in QGHTHXF group（ P 〈 0. 05 ）. Conclusion QGHTHXF has good control effects on the model of rats with nonalcoholic steatohepatitis. The possible mechanism is that it is through adjusting metabolism of fat to alleviate fatty accumulation in liver,inhibit lipid peroxidation and

关 键 词：清肝化痰活血方 非酒精性脂肪性肝炎 胆宁片 大鼠 

分 类 号：R575.5[医药卫生—消化系统]