DNA功能化碳纳米管电极的制备及与青蒿素相互作用的研究  

Preparation of Deoxyribonucleic Acid Functionalized Single-wall Carbon Nanotubes and Study on Interaction with Artemisinin

作　　者：乔雷[1] 张云怀[1] 肖鹏[2] 张怀[1] 李泽全[1] 廖明佳[1] 李静[1] 樊小燕[1] 卢露[1] 

机构地区：[1]重庆大学化学化工学院,重庆400030 [2]重庆大学数理学院,重庆400030

出　　处：《材料导报》2009年第2期17-21,共5页Materials Reports

基　　金：重庆市自然科学基金(CSTS2005BB4200)

摘　　要：单壁碳纳米管(SWCNTs)通过羧基和氨基形成酰胺共价键联上了DNA,从而制得DNA生物功能化的碳纳米管修饰电极。傅立叶红外光谱表明DNA共价结合在SWCNTs上,同时用SEM表征了研究电极的形貌。循环伏安法(CV)研究表明,DNA修饰电极反应过程属于扩散控制过程。与青蒿素的相互作用研究结果表明,SWC-NTs上的DNA分子具有生物活性,且能与其它生物分子发生相互作用。A novel deoxyribonucleic acid（DNA） functionalized single-wall carbon nanotubes（SWCNTs） electrode is preparated in this paper, in which the DNA is linked by forming covalent amide bonds between carboxyl groups of the nanotubes and amino groups at the ends of the DNA oligonucleotides. IR spectra for DNA-modified SWCNTs re- veal DNA is covalently linked to the surface of SWCNTs. SEM micrographs illustrate the morphology and structure of the electrode modified by DNA-SWCNTs. Electrochemical characteristics of DNA-modified SWCNTs are investigated by cyclic voltammetry. The influence of scan rate is examined, and the result suggests that the electrochemical process is controlled by the diffusion for DNA-modified electrode. Additionally, the interaction between DNA molecules modi- fied SWCNTs and artemisinin is studied by cyclic voltammetry, and the results imply that the DNA molecules on the surface of SWCNTs, retaining its bioactivity, still have the ability to interact with other biomolecules.

关 键 词：单壁碳纳米管 DNA 功能化 青蒿素 电化学 

分 类 号：O657[理学—分析化学]