后处理对肺缺血再灌注丙二醛和谷胱甘肽的影响  

Influence of ischemic post-conditioning on malondialdehyde and glutathione during pulmonary ischemic reperfusion

作　　者：罗常进[1] 伍火志[1] 袁茂溪[1] 刘鑫[1] 

机构地区：[1]遵义医学院第五附属医院心胸外科,广东珠海519180

出　　处：《安徽医药》2009年第1期50-51,共2页Anhui Medical and Pharmaceutical Journal

基　　金：遵义医学院珠海校区科研基金项目(NO:KY200610)

摘　　要：目的研究缺血后处理对离体大鼠肺缺血再灌注丙二醛(MDA)和谷胱甘肽(GSH)的影响并分析其可能的作用机制。方法建立离体大鼠肺缺血再灌注损伤模型。12只SD大鼠随机分为2组,每组6只。缺血再灌注(IR)组缺血2 h后以Krebs-Henseleit Buffer(KHB)液再灌注1 h,后处理(IPostC)组缺血2 h后给予重复一次的5 min灌注和5 min缺血的后处理,即以KHB液行再灌注1 h。持续监测及记录肺动脉压(PAP),缺血前及再灌注末分别留取3 ml的灌流液,缺血前、再灌注始及再灌注末分别切取约20 mg肺组织制作10%的组织匀浆,灌流液及肺组织匀浆用于测定MDA及GSH的含量,再灌注结束测定肺湿/干重比(W/D)。结果IPostC组MDA、GSH及W/D较IR组明显降低(P<0.05)。结论缺血后处理能明显减轻离体大鼠肺缺血再灌注损伤。Aim To investigate the influence of ischemic post-conditioning on malondialdehyde and glutathione during ischemic reperfusion of lung in rats. Methods The model of ischemia-reperfusion injury in isolated buffer-perfused rat lungs was prepared. 12 rats were randomly allocated to two groups （6 for each group）. For ischemic reperfusion （IR） group, interrupted perfusion and ventilation were performed for 2 hours and followed by reperfusion for 1 hour. For post-conditioning （IPostC） group,ischemic post-conditioning （5R/5I, twice） was performed between the end of ischemia and the beginning of reperfusion. Pulmonary arterial pressure（PAP） was measured continuously. 3ml perfusate was obtained before ischemia and at the end of reperfusion respectively. Small pieces of lung tissue （ about 20 mg） were cut before ischemia and at the beginning and end of reperfusion and made into homogenate. The concentration of malondialdehyde （MDA） and glutathione （GSH） were determined in perfusate and homogenate. The wet to dry. weight ratio （W/D） were also measured at the end of reperfusion. Results After 2 hours of ischemia and 1 hour of reperfusion, the levels of MDA, GSH and W/D became significantly lower in IPostC group than that in IR group. Conclusion Ischemic post-conditioning can reduce significantly ischemic reperfusion injury in isolated rat lungs.

关 键 词：肺缺血 再灌注 缺血后处理 丙二醛 谷胱甘肽 

分 类 号：R363[医药卫生—病理学]