人胆囊癌肝转移模型的建立及初步的影像学评估  

Establishment and Initial Evaluation of Liver Metastasis Model of Human Gallbladder Carcinoma in Nude Mice

作　　者：锁涛[1] 童洪飞[1] 童赛雄[1] 刘厚宝[1] 沈振斌[1] 艾志龙[1] 凌跃新[1] 

机构地区：[1]复旦大学附属中山医院普外科,上海200032

出　　处：《中国临床医学》2008年第6期793-794,共2页Chinese Journal of Clinical Medicine

摘　　要：目的:建立胆囊癌肝脏转移动物模型,对其进行初步影像学评估,为晚期胆囊癌的各种干预治疗提供实验动物模型及评估工具。方法:用脾内接种法建立人胆囊癌细胞系(GBC-SD)的裸鼠肝脏转移模型。接种后第45天对肝脏行CT检查,并处死裸鼠后取下肝脏行病理检查。结果:组织学检查证实裸鼠的胆囊癌肝转移率为50%(5/10),CT检查肝转移率为40%(4/10),CT检查的阳性率为80%(4/5)。结论:脾脏接种法可以成功建立胆囊癌肝脏转移动物模型,CT可以作为初步评估该模型的影像学工具。Objective：To establish and evaluate liver metastasis model of gallbladder cancer in nude mice for further clinical trial. Methods：The gallbladder carcinoma cells GBC SD were injected into the spleen of nude mouse. After 45 days, the mice were scanned by computerized tomography and sacrificed for pathological examination. Results：The liver metastasis rate was 50 % （5/ 10） pathologically. Four mice with metastasis were detected by CT scan . The positive rate of CT scan was 80 % （4/5）. Conclusion：The results suggest that the liver metastasis model of gallbladder carcinoma in nude mice can be established through spleen injection and evaluated by CT initially.

关 键 词：裸鼠 胆囊癌细胞 肝转移 

分 类 号：R735.88[医药卫生—肿瘤]