基于超氧化物歧化酶/氧化锌的超氧离子传感器  

Superoxide Biosensor Based on Superoxide Dismutase/Nano-ZnO

作　　者：邓子峰[1] 徐伟[1] 杨晓[1] 

机构地区：[1]同济大学化学系,上海200092

出　　处：《高等学校化学学报》2009年第1期37-41,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20643002)资助

摘　　要：利用物理气相沉积法制备了纳米氧化锌(Nano-ZnO)膜,通过扫描电子显微镜(SEM)、紫外-可见分光光度法(UV-Vis)、X射线衍射(XRD)及电化学等方法测定了其物理化学性质.实验结果表明,该Nano-ZnO膜是具有多晶六边形纤维锌矿结构的多孔纳米膜,微粒直径在50～100nm,室温禁带宽度3.37eV.采用浸渍法将超氧化物歧化酶(SOD)直接修饰于Nano-ZnO膜上,制备了SOD修饰电极(SOD/ZnO).通过交流阻抗法(EIS)及循环伏安法(CV)证明了SOD能稳定地吸附在多孔ZnO膜上,并实现了直接电子传递;紫外-可见及红外光谱研究证明吸附在ZnO膜上的蛋白质保持了良好的生物催化活性,并成功地构建了第三代超氧离子(O2-)生物传感器.这种生物传感器有较宽的线性范围(氧化电流:0.24～180×10-6mol/L,还原电流:0.12～250×10-6mol/L)、较低的检测限(氧化电流:2×10-7mol/L,还原电流:1×10-7mol/L)、较快的响应时间(4s)以及较好的稳定性.A nanoporous ZnO film （nano-ZnO） was prepared by physical vapor deposited method and its physical and chemical properties were studied by means of SEM, UV-Vis, XRD and electrochemical methods. The results show that nano-ZnO is a nanoporous film with a polycrystalline hexagonal wurtzite structure. The average diameter of the nanoparticles is about 50-100 nm and its band gap is 3.37 eV. A new superoxide dismutase （SOD） modified nano-ZnO electrode （SOD/ZnO） was prepared by dipping method. SOD was modified stably in the nano-ZnO film confirmed by electrochemical impedance spectroscopy （EIS） and the direct electron transfer could be achieved confirmed by cyclic vohammetry （CV）. Spectrum studies indicated that SOD kept its bio-activities. A novel third-generation O2^- biosensor with high selectivity, quick response time （4 s）, large linear range （anodic current response： 0.24-180 ×10^-6 mol/L; cathodic current response： 0. 12-250 ×10^-6 mol/L） and good sensitivity（anodic： 2×10^-7 mol/L; cathodic： 1 ×10^-7 mol/L） was constructed and its analytical properties were studied.

关 键 词：氧化锌 超氧化物歧化酶 超氧离子 生物传感器 

分 类 号：O657.1[理学—分析化学]