毛细管电泳电致化学发光灵敏检测毒品类生物碱及在尿样分析中的应用  

Sensitive Determination of Drug Alkaloids by Capillary Electrophoresis with Electrochemiluminescence Detection and Its Applications to Analysis of Human Urine

作　　者：刘彦明[1] 田伟[1] 

机构地区：[1]信阳师范学院化学化工学院,信阳464000

出　　处：《高等学校化学学报》2009年第1期51-53,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20575056);河南省高校创新人才培养工程项目(批准号:2005126)资助

摘　　要：A new approach for the determination of methylephedrine hydrochloride(ME),thebaine,codeine phosphate(CP),and acetylcodeine(AC) was established by CE-ECL with ionic liquid(1-butyl-3-methylimidazolium tetrafluoroborate,BMIMBF4). The conditions for the CE separation,ECL detection and the effect of ionic liquid(IL) were systematically investigated. Under the optimal conditions,the four analytes were well separated within 8 min using BMIMBF4 as additive in the electrophoretic buffer. The concentration detection limits of ME,thebaine,CP,and AC were 2.1×10-8,1.4×10-7,6.3×10-8,and 3.6×10-8 mol/L(S/N=3),respectively. The limits of quantitation(LOQ,S/N=10) in real human urine samples were 7.6×10-7 mol/L for ME,3.6 ×10-6 mol/L for thebaine,6.5×10-7 mol/L for CP,and 4.6×10-7 mol/L for AC,respectively. The recoveries of four alkaloids at different levels in human urine samples were between 90.0% and 103.5%. The developed method was successfully applied to the determination of four drug alkaloids in human urine samples.A new approach for the determination of methylephedrine hydrochloride （ ME）, thebaine, codeine phosphate （ CP）, and aeetylcodeine （ AC ） was established by CE-ECL with ionic liquid （ 1-butyl-3-methylimidazolium tetrafluoroborate, BMIMBF4 ）. The conditions for the CE separation, ECL detection and the effect of ionic liquid（IL） were systematically investigated. Under the optimal conditions, the four analytes were well separated within 8 min using BMIMBF4 as additive in the electrophoretic buffer. The concentration detection limits of ME, thebaine, CP, and AC were 2. 1 ×10^-8, 1.4 ×10^-7, 6.3 ×10^-8, and 3.6 ×10^-8 mol/L （S/N=3）, respectively. The limits of quantitation （LOQ, S/N = 10） in real human urine samples were 7.6 × 10^-7 moL/L for ME, 3.6 × 10^-6 mol/L for thebaine, 6.5 × 10^-7 mol/L for CP, and 4. 6 × 10^-7 mol/L for AC, respectively. The recoveries of four alkaloids at different levels in human urine samples were between 90. 0% and 103.5%. The developed method was successfully applied to the determination of four drug alkaloids in human urine samples.

关 键 词：毛细管电泳 电致化学发光检测 毒品类生物碱 离子液体 尿样分析 

分 类 号：O657[理学—分析化学]