缺氧诱导因子-1α基因沉默对食管鳞癌细胞体外生长的影响  

Effect of hypoxia-inducible factor-1 alpha gene silencing on the growth of human esophageal squamous cancerous cells in vitro

作　　者：肖斌[1] 施瑞华[2] 郝波[2] 林艳[2] 

机构地区：[1]南京医科大学第一附属医院老年医学科,210029 [2]南京医科大学第一附属医院消化科,210029

出　　处：《实用医学杂志》2009年第2期177-179,共3页The Journal of Practical Medicine

基　　金：江苏省自然科学基金资助项目(编号:BK2005153)

摘　　要：目的:探讨食管鳞癌细胞中缺氧诱导因子-1α(HIF-1α)基因与食管鳞癌细胞增殖活力和细胞周期的关系。方法:以RNA干扰方法构建出HIF-1α基因沉默食管鳞癌细胞株,通过Westernblot检测HIF-1α基因在细胞中的表达,通过流式细胞术分析了解HIF-1α基因沉默后细胞周期的变化,通过细胞增殖实验观察HIF-1α基因沉默细胞与对照细胞、模拟缺氧细胞增殖活性的差异。结果:细胞增殖实验发现沉默HIF-1α基因后的Eca-109细胞增殖活力较对照细胞明显低下,为对照细胞的62.6%(0.4768±0.1743vs0.7611±0.2165,P=0.012),流式细胞仪分析表明,HIF-1α基因沉默后的Eca-109细胞与对照细胞相比,G1/G0期细胞明显增加(P<0.05),G2/M期细胞变化不大,S期细胞比例明显减少。结论:核糖核酸干扰HIF-1α的表达后,能抑制鳞癌细胞的增殖活力,并可能阻滞肿瘤细胞周期,抑制HIF-1α的表达,有可能导致肿瘤生长的抑制。Objective To investigate the association of hypoxia-inducible factor-1 alpha （HIF-1α ） gene with the proliferation and cycle of human esophageal squamous cancerous cells. Methods The silencing of HIF-1α was constructed by RNA interference in human esophageal squamous cancerous cell Eca-109. HIF-1α expression was detected by Western blot. The changes in the cycle of the cells with HIF-1α gene silencing were determined by flow cytometry （FCM）. The differences in the proliferation of the cells with HIF-1α gene silencing, the control cells, and hypoxia-imitated cells were observed. Results The proliferative activity of the Eca-109 cells with HIF-1α gene silencing was markedly reduced as compared with the control cells （0.4768 ± 0.1743 vs 0.761 1±0.216 5, P = 0.012）. FCM showed that HIF-1α inhibition caused cell cycle arrest in G1/G0 phase （P 〈 0.05）. Conclusion Silencing of HIF-1α by RNA interference can inhibit the proliferative activity of the squamous cancerous cell line Eca-109 and arrest the cells in G1/G0 phase.

关 键 词：食管肿瘤 RNA干扰 缺氧诱导因子-1Α 

分 类 号：R735.1[医药卫生—肿瘤]