原发性肝细胞癌患者肝组织中乙型肝炎病毒X基因的变异  

The mutation of hepatitis B virus X gene in the liver tissues of hepatocellular carcinoma patients

作　　者：李伟[1] 刘传苗[1] 赵守松[1] 

机构地区：[1]蚌埠医学院第一附属医院感染科,233000

出　　处：《实用医学杂志》2009年第2期180-182,共3页The Journal of Practical Medicine

基　　金：安徽省卫生厅课题(编号:06B080)

摘　　要：目的:对原发性肝细胞癌(hepatocellular carcinoma,HCC)患者肝组织中乙型肝炎病毒(hepatitis Bvirus,HBV)X基因及其常见变异位点进行检测分析,以探讨HBVX基因及其变异与HCC发生发展的关系。方法:采用聚合酶链反应(polymerase chain reaction,PCR)方法检测22例乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)阳性的HCC患者肝组织中HBVX基因,并对PCR产物进行基因测序分析,同时检测5例正常肝脏组织中HBVX基因。结果:22例HCC患者癌组织及癌周组织中HBVX基因检出率分别为68%和77%,5例正常肝脏组织中均未检测到HBVX基因,癌组织及癌周组织中HBVX基因检出率均很高(均P<0.0125),而癌组织与癌周组织的阳性率没有明显差异(P>0.0125)。癌组织及癌周组织中HBVX基因发生1762T/1764A双突变率分别为93%和47%,肝癌组织中双突变率要明显高于癌周组织(P<0.05),未发现1762T及1764A单独发生突变。结论:HCC患者肝组织中HBVX基因检出率很高,肝癌组织中发生1762T/1764A双突变的频率很高,HBVX基因及1762T/1764A双突变与HCC的发生可能有重要关系。Objective To explore the association of hepatitis B virus （HBV） X gene and its mutation with hepatoeellular carcinogenesis by detecting HBV X gene and its frequent mutation sites in the liver tissues of hepatocellular carcinoma （HCC） patients. Methods HBV X genes were detected in 22 HBsAg-positive patients with HHC and 5 HBsAg-negative healthy subjects using polymerase chain reaction （PCR）. The obtained PCR products were then sequenced. Results The detection rate of HBV X gene was high in patients with HHC, 68% in the cancer tissues and 77% in the adjacent tissues （P 〈 0.012 5 for both comparisons）, but the positive rate of X gene did not differ significantly between the caner tissues and the surrounding tissues （P 〉 0.012 5）. The rate of HBV 1762T/1764A double mutation was markedly higher in the cancer tissues than in the adjacent tissues （93% vs 47%, P 〈 0.05）. No 1762T or t764A mutation alone was found. HBV X geue was undetectable in 5 normal liver tissue specimens. Conclusions The detection rate of HBV X gene and the rate of 1762T/1764A double mutation were significantly high in patients withHCC. HBV X gene and the double mutation may play an important role in hepatoeellular carcinogenesis.

关 键 词：肝肿瘤 乙型肝炎病毒X基因 变异 

分 类 号：R735.7[医药卫生—肿瘤] R373.1[医药卫生—临床医学]