脉冲磁场对白血病细胞HL60/ADR多药耐药的逆转效果及分子机制研究  

Mechanism of pulsed magnetic field on reversal of multi-drug resistance of the leukemic cell HL60/ADR

作　　者：韩秋森[1] 刘晓秋[1] 韩英[1] 赵阿津[1] 宋保民[1] 

机构地区：[1]中国医学科学院北京协和医学院放射医学研究所,天津300192

出　　处：《现代生物医学进展》2009年第1期1-4,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(30570437)

摘　　要：目的:探讨脉冲磁场(pulsed magnetic fields,PMF)对白血病细胞HL60/ADR多药耐药的逆转效果及其可能的分子机制。方法:生长曲线法检测PMF的细胞毒性;MTT法测定HL60、HL60/ADR的IC50及经不同参数PMF作用后HL60/ADR的IC50,计算耐药倍数和逆转倍数;流式细胞术检测PMF作用前后HL60/ADR细胞内药物蓄积变化及多药耐药相关蛋白(Multidrug Resis-tance Related Protein1 MRP1)阳性表达率的变化;RT-PCR法检测MRP1基因表达的变化。结果:单独PMF对HL60/ADR细胞的生长没有明显抑制作用,不同参数(频率为150Hz和250Hz,场强均为40mT,照射时间30min和1h)的PMF均能有效逆转HL60/ADR的多药耐药,150Hz/1h作用最为明显,逆转倍数为5.891倍。PMF作用后Rh123在HL60/ADR细胞内的蓄积增加了近8.0%,而MRP1基因表达下调了68.3%,蛋白阳性表达率下调23.6%。结论:PMF能够通过下调MRP1基因和蛋白的表达,进而增加Rh123在细胞内的蓄积,功能性部分逆转白血病细胞的多药耐药。Objective： To explore effect of Retinoic Acid （RA） on proliferation of human osteosarcoma MG-63 cells and its mechanism of antitumor. Methods： The MG-63 cells treated by 1μmol/L RA were subjected to growth curve counts, flow cytometry, immuncocytochemical assay and light microscopy.We investigated the influence of RA on proliferation,cellcycle,expression of associated oncogene and tumor suppressor gene of MG-63cells. Then we analyzed the mechanism of RA in inducing differentiation primarily. Results： Seven days after treated by 1 μmol/LRA, the inhibition rate of proliferation of MG-63 cells amounted to 42.2 % and RA reduced the rate of progression from G1 to S phase.The cells tended to be flat and spread. Meanwhile,the expression level of oncogene was downregulated and the expression level of tumor suppressor gene was upregulated. Conclusion： 1 μmol/L RA could inhibit proliferation of MG-63cells, reverse the malignant phenotype characters, downregulate expression of oncogene c-Myc, c-fos and upregulate expression of Tumor Suppressor Gene Rb, p27, as a result,induce MG-63 cells into differentiation effectively.

关 键 词：白血病 多药耐药 逆转 脉冲磁场 HL60/ADR 

分 类 号：R733.71[医药卫生—肿瘤] Q64[医药卫生—临床医学]