食管鳞状细胞癌中热休克蛋白90α和基质金属蛋白酶-2的表达及意义  

Expression and clinical significance of HSP 90α and MMP - 2 in esophageal squamous cell carcinoma

作　　者：许建功[1] 李庆军[1] 赵宾[1] 

机构地区：[1]新乡医学院第三附属医院胸外科,453000

出　　处：《中国实用医刊》2009年第2期14-18,共5页Chinese Journal of Practical Medicine

摘　　要：目的研究热休克蛋白90α（HSP90α）和基质金属蛋白酶-2（MMP-2）在食管鳞状细胞癌（简称鳞癌）中的蛋白表达，分析二者表达的相关性及其与临床病理特征之间的关系。方法采用免疫组织化学Elivision非生物素检测方法．对58例食管鳞癌、30例癌旁正常食管上皮组织切片进行染色，观察、评定HSP90α和Survivin蛋白表达的阳性率及表达强度。结果HSP90α阳性产物主要位于细胞核，在食管鳞癌及食管正常上皮的阳性表达率分别为68．97％和13．33％。MMP-2阳性产物主要位于细胞核，在食管鳞癌及食管断端正常上皮的阳性表迭率分别为70．69％和16．67％。Survivin阳性产物主要位于细胞核，在食管鳞癌及食管断端正常上皮的阳性表达率分别为56．90％和6．67％。在食管鳞癌中，HSP90α表达与MMP-2表达呈正相关（rs=0．534，P=0，000）。结论HSP90α与MMP-2在食管鳞癌的发生、发展中起重要作用。Objective To study the expression of HSP 90α and MMP- 2 proteins in esophageal squamous cell oarcinoma（ESCC） , and to analyze the relationships among expression, between protein expression and clinicopathologic factors. Methods Fifty - eight cases of ESCCs ,30 cases of pericarcinoma normal tissues Were immunohistochemically stained using HSP 90α, MMP -2 and Survivin monoclonal antibodies with ElivisionTM plus non -biotin detection system, the positive expression rate and expression intensity were evaluated. Results The HSP 90α protein positive expression rates were 68.97% and 13.33% in ESCC and normal epithelium respectively that mainly being expressed in nucleus, and the MMP - 2 protein positive expression rates were 70.69% and 16.67% in ESCC and normal epithelium respectively that mainly being expressed in nucleus. HSP 90α protein expression was positively correlated with MMP - 2. Conclusions HSP 90α and MMP - 2 may play important roles in the carcinogenesis and development of ESCC.

关 键 词：食管鳞癌 热休克蛋白90Α 基质金属蛋白酶-2 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]